RayBio® C-Series Human Inflammation Antibody Array 1 Kit. Detects 20 Human Inflammatory Factors. Suitable for all liquid sample types.
Human Inflammation Array C1
Human Inflammation Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| GCSF
| GM-CSF
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
|
IL-6
| IL-7
| IL-8 (CXCL8)
| IL-10
| IL-11
|
IL-12 p40
| IL-12 p70
| IL-13
| I-309 (TCA-3/CCL1)
| TIMP-2
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Inflammation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Human endoderm stem cells reverse inflammation-related acute liver failure through cystatin SN-mediated inhibition of interferon signaling
- Species:
- Human
- Sample Type:
- human endoderm stem cells (hEnSCs), mesenchymal stem cells (MSCs)
- Year:
- 2023
- Journal Name:
- Cell Research
MiR-146a-5p enrichment in small-extracellular vesicles of relapsed pediatric ALCL patients promotes macrophages infiltration and differentiation
- Species:
- Human
- Sample Type:
- THP-1 cell
- Year:
- 2023
- Journal Name:
- Biochemical Pharmacology
Alcohol consumption modulates Candida albicans-induced oral carcinogenesis and progression.
- Species:
- Human
- Sample Type:
- Ca9-22 cell
- Year:
- 2023
- Journal Name:
- Journal of Oral Biosciences
New insights into cholesterol-mediated ERRα activation in breast cancer progression and pro-tumoral microenvironment orchestration
- Species:
- Human
- Sample Type:
- MCF-7 cell, MDA-MB-231 cell
- Year:
- 2022
- Journal Name:
- Febs Journal
The impact of pulmonary tuberculosis on immunological and metabolic features of diabetic patients
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Frontiers in immunology
Spent culture supernatant of Streptococcus gordonii mitigates inflammation of human periodontal cells and inhibits proliferation of pathogenic oral microbes
- Species:
- Human
- Sample Type:
- human gingival fibroblasts (HGFs)
- Year:
- 2022
- Journal Name:
- Journal of Periodontology
Upregulation of Circ_0035266 Contributes to the Malignant Progression of Inflammation-Associated Malignant Transformed Cells Induced by Tobacco-Specific Carcinogen NNK
- Species:
- Human
- Sample Type:
- BEAS-2B cell
- Year:
- 2022
- Journal Name:
- Toxicological Sciences
Photoreceptor cells produce inflammatory products that contribute to retinal vascular permeability in a mouse model of diabetes.
- Species:
- mouse
- Sample Type:
- Conditioned Media (retinal endothelial cells)
- Year:
- 2017
- Journal Name:
- Diabetologia
Muscadine grape seed oil as a novel source of tocotrienols to reduce adipogenesis and adipocyte inflammation
- Species:
- Human
- Sample Type:
- Conditioned Media (Adipose derived stem cells treated with muscadine grape seed oil)
- Year:
- 2015
- Journal Name:
- Food and Function
- PubMed ID:
- 26073057
Maslinic Acid enhances signals for the recruitment of macrophages and their differentiation to m1 state
- Species:
- Human
- Sample Type:
- Conditioned Media (THP1 human macrophages stimulated with LPS); Tissue Lysate (Plasma and placental tissue Lysates)
- Year:
- 2015
- Journal Name:
- Evidence-based complementary and alternative medicine : eCAM
- PubMed ID:
- 25821495