Membrane-based inflammation cytokine array detects 40 human inflammatory factors.
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Human Inflammation Array C3
Human Inflammation Array C3
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 40 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| GCSF
| GM-CSF
| ICAM-1 (CD54)
|
| IFN-gamma
| I-309 (TCA-3/CCL1)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
|
| IL-3
| IL-4
| IL-6
| IL-6 R
| IL-7
|
| IL-8 (CXCL8)
| IL-10
| IL-11
| IL-12 p40
| IL-12 p70
|
| IL-13
| IL-15
| IL-16
| IL-17A
| IP-10 (CXCL10)
|
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| M-CSF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
|
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| RANTES (CCL5)
| TGF beta 1
| TNF alpha
|
| TNF beta (TNFSF1B)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| PDGF-BB
| TIMP-2
|
Typical Data
Kit Components
- Human Inflammation Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancer
- Species:
- Human
- Sample Type:
- PC3 cell, METTL1 cell
- Year:
- 2023
- Journal Name:
- Molecular Cancer
GRB2 stabilizes RAD51 at reversed replication forks suppressing genomic instability and innate immunity against cancer
- Year:
- 2024
- Journal Name:
- Nature Communications
- PubMed ID:
- 38459011
Inactivation of ATG13 stimulates chronic demyelinating pathologies in muscle-serving nerves and spinal cord
- Year:
- 2025
- Journal Name:
- Immunologic Research
- PubMed ID:
- 39777574
A blood glucose fluctuation-responsive delivery system promotes bone regeneration and the repair function of Smpd3-reprogrammed BMSC-derived exosomes
- Year:
- 2024
- Journal Name:
- International journal of oral science
- PubMed ID:
- 39616150
Adalimumab Effectively Decreases Inflammation Downstream of TNFα Signaling in Synoviocytes from Extended Oligoarticular Juvenile Idiopathic Arthritis
- Year:
- 2024
- Journal Name:
- Rheumatology and therapy
- PubMed ID:
- 38070102
A tumor suppressor protein encoded by circKEAP1 inhibits osteosarcoma cell stemness and metastasis by promoting vimentin proteasome degradation and activating anti-tumor immunity
- Year:
- 2024
- Journal Name:
- Journal of experimental & clinical cancer research : CR
- PubMed ID:
- 38383479
Therapeutic potency of compound RMY-205 for pulmonary fibrosis induced by SARS-CoV-2 nucleocapsid protein
- Species:
- Human
- Sample Type:
- HFL-1 cell
- Year:
- 2023
- Journal Name:
- Cell Chemical Biology
Cerebral microvascular endothelial cell-derived extracellular vesicles regulate blood - brain barrier function
- Year:
- 2023
- Journal Name:
- Fluids and barriers of the CNS
- PubMed ID:
- 38114994
Crystal structure of Leptospira LSS_01692 reveals a dimeric structure and induces inflammatory responses through Toll-like receptor 2-dependent NF-κB and MAPK signal transduction pathways
- Year:
- 2023
- Journal Name:
- Febs Journal
- PubMed ID:
- 37243454
ENOX2 inhibition enhances infiltration of effector memory T-cell and mediates response to chemotherapy in immune-quiescent nasopharyngeal carcinoma
- Species:
- Human
- Sample Type:
- NPC43 cell
- Year:
- 2023
- Journal Name:
- Journal of advanced research
