Glass slide multiplex ELISA array quantitatively detects 10 human inflammatory factors.
Human Inflammation Array Q1
Human Inflammation Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation, JAK/STAT Signaling, MAPK Signaling, NFkB Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
| IL-13
|
IL-4
| IL-6
| IL-8 (CXCL8)
| MCP-1 (CCL2)
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Inflammation Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-INF-1.gal)
Excel (QAH-INF-1_Analysis_Tool.xls)
citations
Establishment and Optimization of a Human Flow-Based Hollow Fiber In Vitro Blood-Brain Barrier Model for Systemic Inflammatory Responses
- Year:
- 2026
- Journal Name:
- Pharmaceutics
- PubMed ID:
- 42514895
Sele-targeted siRNA liposome nanoparticles inhibit pathological scars formation via blocking the cross-talk between monocyte and endothelial cells: a preclinical study based on a novel mice scar model
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatant of endothelial-monocyte co-culture)
- Year:
- 2024
- Journal Name:
- Journal of Nanobiotechnology
- PubMed ID:
- 39593088
Cardiac tissue model of immune-induced dysfunction reveals the role of free mitochondrial DNA and the therapeutic effects of exosomes
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (I3M medium from the cardiac tissue compartment)
- Year:
- 2024
- Journal Name:
- Science Advances
- PubMed ID:
- 38536913
A personalized osteoarthritic joint-on-a-chip as a screening platform for biological treatments
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell culture medium of the chips); Other Body Fluid (synovial fluids)
- Year:
- 2024
- Journal Name:
- Materials Today Bio
- PubMed ID:
- 38757057
Aberrant Activation of the Hedgehog Pathway in Cutaneous Melanoma: Therapeutic Potential of Pharmacological Inhibitors
- Year:
- 2026
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 41596411
Synoviocyte-chondrocyte triculture model for early-stage PTOA: fibronectin fragment-induced catabolic effects in vitro and in vivo
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture supernatants of fnf-treated macrophages, PTOA triculture media)
- Year:
- 2025
- Journal Name:
- Frontiers in Bioengineering and Biotechnology
- PubMed ID:
- 41425156
Prognostic impact of the lymph node yield on survival in patients with stage I lung adenocarcinoma receiving sublobar resection
- Species:
- Human
- Sample Type:
- Serum (pre-surgery and postoperative day 1)
- Year:
- 2026
- Journal Name:
- Frontiers in Molecular Biosciences
- PubMed ID:
- 41953047
Transport of Non-Steroidal Anti-Inflammatory Drugs across an Oral Mucosa Epithelium In Vitro Model
- Species:
- Human
- Sample Type:
- Other / Unspecified (apical, basolateral media)
- Year:
- 2024
- Journal Name:
- Pharmaceutics
- PubMed ID:
- 38675204
Low Immunogenicity of Keratinocytes Derived from Human Embryonic Stem Cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatant from co-culture system)
- Year:
- 2024
- Journal Name:
- Cells
- PubMed ID:
- 39273019
CSF Findings in Chinese Patients with NMDAR, LGI1 and GABABR Antibody-Associated Encephalitis
- Species:
- Human
- Sample Type:
- Serum (autoimmune encephalitis patients)
- Year:
- 2024
- Journal Name:
- Journal of Inflammation Research
- PubMed ID:
- 38523682