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Human MMP-2 ELISA Kit Standard Curve
Human MMP-2 ELISA Kit
RayBio® Human MMP-2 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 3,500 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 3.5 ng/ml - 800 ng/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 3, -7, -8, -10, -12, -13, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 5 - 50 fold |
| Accession Number | P08253 |
| Gene Id | 4313 |
| Gene Symbols | MMP2, CLG4A |
| Protein Name / Synonyms | 72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) (TBE-1) [Cleaved into: PEX] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human MMP-2 qIPCR ELISA Kit
As low as $749.00
RayPlex® Human MMP-2 Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human MMP-2 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified MMP-2 (MMP2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Cosmeceutical application of extracts from the flowers, stems, and leaves of Buddleja davidii grown at different altitudes
- Species:
- Human
- Sample Type:
- Other / Unspecified (CCD-966SK cells)
- Year:
- 2025
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 40417216
Anti-Metastatic Potential of Hydroalcoholic Extract of Pistacia Khinjuk Leaves and Its Mechanism: An in Vitro Study
- Species:
- Human
- Sample Type:
- Other / Unspecified (secretions of cells treated with the extract)
- Year:
- 2025
- Journal Name:
- Journal of Advances in Medical and Biomedical Research
Prenatal exposure to Benzoampyrene affects maternal-fetal outcomes via placental apoptosis
- Species:
- Human
- Sample Type:
- Other / Unspecified (placenta)
- Year:
- 2024
- Journal Name:
- Scientific Reports
- PubMed ID:
- 39043924
A Panel of Potential Serum Markers Related to Angiogenesis, Antioxidant Defense and Hypoxia for Differentiating Cutaneous Squamous Cell Carcinomas from Actinic Keratoses
- Species:
- Human
- Sample Type:
- Serum (cSCC and actinic keratosis patients)
- Year:
- 2024
- Journal Name:
- Journal of Personalized Medicine
- PubMed ID:
- 38248804
Alcohol consumption modulates Candida albicans-induced oral carcinogenesis and progression.
- Species:
- Human
- Sample Type:
- Ca9-22 cell
- Year:
- 2023
- Journal Name:
- Journal of Oral Biosciences
The Inflammatory Profile Orchestrated by Inducible Nitric Oxide Synthase in Systemic Lupus Erythematosus
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of Personalized Medicine
Primmorph extracts and mesohyls of marine sponges inhibit proliferation and migration of hepatocellular carcinoma cells in vitro
- Species:
- Human
- Sample Type:
- Other / Unspecified (HepG2 cells)
- Year:
- 2019
- Journal Name:
- Journal of Pharmaceutical Analysis
- PubMed ID:
- 31452967
Hemimycale Arabica Induced Non-Cytotoxic Anti-Migratory Activity in Hepatocellular Carcinoma In Vitro
- Species:
- Human
- Sample Type:
- HepG2 cell
- Year:
- 2022
- Journal Name:
- Asian Pacific Journal of Cancer Prevention
The FoxP1 gene regulates lung function, production of matrix metalloproteinases and inflammatory mediators, and viability of lung epithelia
- Species:
- Human
- Sample Type:
- BEAS-2B cell
- Year:
- 2022
- Journal Name:
- Respiratory Research
Tamoxifen/raloxifene loaded liposomes for oral treatment of breast cancer
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2020
