Quantibody® Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs. Suitable for all liquid sample types.
Human MMP Array Q1
Human MMP Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
MMP-1
| MMP-10
| MMP-13
| MMP-2
| MMP-3
|
MMP-8
| MMP-9
| TIMP-1
| TIMP-2
| TIMP-4
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human MMP Array Q1 Slide(s)
- Sample Diluent
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-MMP-1.gal)
Excel (QAH-MMP-1_Analysis_Tool.xls)
citations
Scar-Degrading Endothelial Cells as a Treatment for Advanced Liver Fibrosis
- Species:
- Human
- Sample Type:
- liver sinusoidal endothelial cells (LSECs)
- Year:
- 2022
- Journal Name:
- Advanced Science
Androgen Receptor Activation Induces Senescence in Thyroid Cancer Cells
- Species:
- Human
- Sample Type:
- 8505C cell
- Year:
- 2023
- Journal Name:
- Cancers
3D extracellular matrix microenvironment in bioengineered tissue models of primary pediatric and adult brain tumors.
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2019
- Journal Name:
- Nature Communications
A Preliminary Direct Comparison of the Inflammatory Reduction and Growth Factor Production Capabilities of Three Commercially Available Wound Products: Collagen Sheet, Manuka Honey Sheet, and a Novel Bioengineered Collagen Derivative + Manuka Honey + Hydroxyapatite Sheet
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
Human pericytes adopt myofibroblast properties in the microenvironment of the IPF lung
- Species:
- Human
- Sample Type:
- Conditioned Media (Human Pericytes)
- Year:
- 2017
- Journal Name:
- JCI insight
- PubMed ID:
- 29263297
Decreased stathmin-1 expression inhibits trophoblast proliferation and invasion and is associated with recurrent miscarriage
- Species:
- Human
- Sample Type:
- Conditioned Media (HTR-8 cells treated with siRNAs for Stathmin-1, invasive extravillous trophoblasts)
- Year:
- 2015
- Journal Name:
- American Journal of Pathology
- PubMed ID:
- 26272359
Peripheral blood monocyte subsets predict antiviral response in chronic hepatitis C.
- Species:
- Human
- Sample Type:
- Conditioned Media (peripheral monocytes )
- Year:
- 2011
- PubMed ID:
- 21848603
Role of plasma matrix-metalloproteases (MMPs) and their polymorphisms (SNPs) in sepsis development and outcome in ICU patients
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2014
- Journal Name:
- Scientific reports
Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2
- Species:
- Human
- Sample Type:
- Conditioned Media (MDA-MB-231 cells)
- Year:
- 2014
- Journal Name:
- Journal of Molecular Signaling
Matrix Metalloproteases and Tissue Inhibitors of Metalloproteinases in Medial Plica and PannuslikeTissue Contribute to Knee Osteoarthritis Progression
- Species:
- Human
- Sample Type:
- Tissue Lysate (Medial plica and pannus tissue from OA patients)
- Year:
- 2013
- Journal Name:
- Plos One