Human MMR ELISA Kit

Référence ELH-MMR-1

Conditionnement : 1PlateKit

Marque : RayBiotech

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Téléphone : +1 850 650 7790

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

Human MMR ELISA Kit

RayBio® Human MMR (CD206/MRC1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
0.41 ng/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
0.41 ng/ml - 100 ng/ml
Specificity
This ELISA antibody pair detects human MMR. Other species not determined.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
30 fold
Accession Number
P22897
Gene Id
4360
Gene Symbols
MRC1, CLEC13D, CLEC13DL, MRC1L1
Protein Name / Synonyms
Macrophage mannose receptor 1 (MMR) (C-type lectin domain family 13 member D) (C-type lectin domain family 13 member D-like) (Macrophage mannose receptor 1-like protein 1) (CD antigen CD206)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Shipping TypeBlue ice
Storage≤-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Customize This Human MMR ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified MMR (CD206 / MRC1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Secretory IgA Is a Key Marker Among Gut Barrier Dysfunction-Related Immunoglobulins Predicting Outcomes in ACLF

    Species:
    Human
    Sample Type:
    Serum (cirrhosis patients, ACLF development)
    Year:
    2025
    Journal Name:
    Liver International
    PubMed ID:
    40944472
  • Radiosensitizing effect of astaxanthin on radiation-induced cytotoxicity in breast cancer cells

    Species:
    Human
    Sample Type:
    Cell Lysate (treated, control cell lysate); Tissue Lysate / Homogenate
    Year:
    2026
    Journal Name:
    Journal of the Egyptian National Cancer Institute
    PubMed ID:
    42252369
  • Cathepsin K regulates the tumor growth and metastasis by IL-17/CTSK/EMT axis and mediates M2 macrophage polarization in castration-resistant prostate cancer

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    Cell Death & Disease
  • Characterizing the lavage and serum cytokine profiles of interstitial pneumonia with autoimmune features and their implications for progressive fibrosis

    Species:
    Human
    Sample Type:
    BALF, serum
    Year:
    2023
    Journal Name:
    Rheumatology
  • Bi- and tri-valent T cell engagers deplete tumour-associated macrophages in cancer patient samples

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (malignant ascites supernatant)
    Year:
    2019
    Journal Name:
    Journal for Immunotherapy of Cancer
    PubMed ID:
    31753017
  • Influence of NAFLD and bariatric surgery on hepatic and adipose tissue mitochondrial biogenesis and respiration

    Species:
    Human
    Year:
    2022
  • Increased Levels of Soluble CD206 Associated with Rapidly Progressive Interstitial Lung Disease in Patients with Dermatomyositis

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2020
    Journal Name:
    Mediators of Inflammation
  • Liquid biomarkers of macrophage dysregulation and circulating spike protein illustrate the biological heterogeneity in patients with post-acute sequelae of COVID-19

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2022
    Journal Name:
    Journal of Medical Virology
  • Soluble CD206 levels correlate with disease deterioration and predict prognosis of anti-MDA5 antibody-positive dermatomyositis related interstitial lung disease

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Clinical Respiratory Journal

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