Human Monkeypox Virus (MPXV) IgG ELISA Kit

Principle of the Assay: This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the Monkeypox Virus (MPXV) IgG in the sample. The micro ELISA plate provided in this kit is pre-coated with purified MPXV antigen, after adding samples to wells, the MPXV IgG in the samples will combine with the pre-coated MPXV antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated goat anti human IgG to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain MPXV IgG and HRP conjugated anti-human IgG will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether MPXV IgG exists in the tested samples or not.

Intended Uses: This ELISA kit applies to the in vitro qualitative determination of Monkeypox Virus (MPXV) IgG in serum or plasma.

Small volumes of MPXV elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2569817 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Monkeypox Virus (MPXV) IgG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MPXV. The ELISA analytical biochemical technique of the MBS2569817 kit is based on MPXV antibody-MPXV antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MPXV antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MPXV. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).