TheHuman NOTCH1 / DLL4 Ligand Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between NOTCH1 and DLL4 in the presence of potential inhibitors within 4 hours.
Human NOTCH1 / DLL4 Ligand Binding Assay Kit
Human NOTCH1 / DLL4 Ligand Binding Assay Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Compatible Sample Types | Plasma, Serum, Antibodies, Proteins, Small Molecules, Peptides |
| Accession Number | P46531 / Q9NR61 |
| Gene Id | 4851 / 54567 |
| Gene Symbols | NOTCH1 TAN1 / DLL4 UNQ1895/PRO4341 |
| Protein Name / Synonyms | Neurogenic locus notch homolog protein 1 (Notch 1) (hN1) (Translocation-associated notch protein TAN-1) [Cleaved into: Notch 1 extracellular truncation (NEXT) Notch 1 intracellular domain (NICD)] / Delta-like protein 4 (Drosophila Delta homolog 4) (Delta4) |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Research Area | Receptor-Ligand Binding |
| Shipping Type | Blue ice |
| Storage | 4°C |
Kit Components
- Pre-coated 96-well microplate
- Wash Buffer
- Assay Diluent
- Ligand Protein
- Detection Antibody
- HRP Conjugated Anti-IgG
- TMB One-Step Substrate Reagent
- Stop Solution
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Shaker
- Pipettes capable of accurately delivering 2 µL to 1 mL volumes
- Pipettes capable of delivering 1-25 mL volumes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Distilled or deionized water
- Tubes to prepare sample dilutions
Assay Procedure Summary
- Prepare all reagents and test reagent samples as instructed.
- Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
- Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
- Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
- Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.
Storage/Stability
The kit may be stored at 4°C up to 1 month from the date of shipment. Shelf life can be extended up to 6 months by storing protein and antibodies, at -20°C immediately upon delivery. All other kit components should be stored at 4°C.