RayBio® Human Osteopontin (SPP1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human Osteopontin (OPN) ELISA Kit
Human Osteopontin (OPN) ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 50 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 50 pg/ml - 18000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the following cytokines tested (human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 3 - 100 fold |
| Accession Number | P10451 |
| Gene Id | 6696 |
| Gene Symbols | SPP1, BNSP, OPN, PSEC0156 |
| Protein Name / Synonyms | Osteopontin (Bone sialoprotein 1) (Nephropontin) (Secreted phosphoprotein 1) (SPP-1) (Urinary stone protein) (Uropontin) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Osteopontin (OPN) ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Osteopontin (SPP1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Osteopontin: A novel marker of pre-symptomatic sporadic Alzheimer's disease
- Species:
- Human
- Sample Type:
- Other / Unspecified (frontal cortex)
- Year:
- 2024
- Journal Name:
- Alzheimer's & dementia : the journal of the Alzheimer's Association
- PubMed ID:
- 39072932
Osteopontin as a Tumor Marker in Ovarian Cancer
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Journal of mid-life health
Decreased Levels of Microfibril-Associated Glycoprotein (MAGP)-1 in Patients with Colon Cancer and Obesity Are Associated with Changes in Extracellular Matrix Remodelling
- Species:
- Human
- Sample Type:
- Whole Blood (circulating blood)
- Year:
- 2021
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 34445187
Betulin Promotes Differentiation of Human Osteoblasts In Vitro and Exerts an Osteoinductive Effect on the hFOB 1.19 Cell Line Through Activation of JNK, ERK1/2, and mTOR Kinases
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell culture media of hFOB 1.19 cells)
- Year:
- 2019
- Journal Name:
- Molecules
- PubMed ID:
- 31331121
Diagnostic Value of Multiple Serum Biomarkers for Vancomycin-Induced Kidney Injury
- Species:
- Human
- Sample Type:
- Serum (VIKI, non-VIKI and healthy controls)
- Year:
- 2021
- Journal Name:
- Journal of Clinical Medicine
- PubMed ID:
- 34768522
Markers of increased cardiovascular risk in patients with chronic kidney disease
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2014
- Journal Name:
- Lipids in Health and Disease
OSTEOPONTIN AND OSTEOPROTEGERIN AS POTENTIAL BIOMARKERS IN ABDOMINAL AORTIC ANEURYSM BEFORE AND AFTER TREATMENT
- Species:
- Human
- Sample Type:
- Serum (Fasting Blood Sample)
- Year:
- 2014
- Journal Name:
- International scholarly research notices
Osteopontin as a Marker for Response to Pegylated Interferon Alpha-2b Treatment in Chronic HCV Saudi Patients
- Species:
- Human
- Sample Type:
- Serum (Chronic HCV patients)
- Year:
- 2014
- Journal Name:
- Wulfenia Journal
Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study
- Species:
- Human
- Sample Type:
- Serum (Lupus Nephritis patients)
- Year:
- 2016
- Journal Name:
- Journal of Proteome Research
- PubMed ID:
- 27211902
Alfa-Fetoprotein L3 Subfraction and Osteopontin: Novel Markers for the Diagnosis of Hepatocellular Carcinoma
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2013
- Journal Name:
- Journal of American Science
