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Human P-Selectin ELISA Kit
Human P-Selectin ELISA Kit
RayBio® Human P-Selectin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 20 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 20 pg/ml - 30 ng/ml |
| Specificity | This ELISA kit shows no significant cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, EGF, ENA-78, EOT, FGF-4, FGF-6, FGF-7, IL-1 alpha, IL-1 beta, IL-4, IL-5, IL-11, IL-12 p70, IL-12 p40, IL-13, I-309, IP-10, M-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TIMP-1, TIMP-2, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 50 - 500 fold |
| Accession Number | P16109 |
| Gene Id | 6403 |
| Gene Symbols | SELP, GMRP, GRMP |
| Protein Name / Synonyms | P-selectin (CD62 antigen-like family member P) (Granule membrane protein 140) (GMP-140) (Leukocyte-endothelial cell adhesion molecule 3) (LECAM3) (Platelet activation dependent granule-external membrane protein) (PADGEM) (CD antigen CD62P) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human P-Selectin qIPCR ELISA Kit
As low as $749.00
RayPlex® Human P-selectin Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human P-Selectin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified P-Selectin (SELP), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Predictive indicators in peripheral blood and left atrium blood for left atrial spontaneous echo contrast in atrial fibrillation patients
- Species:
- Human
- Sample Type:
- Plasma (plasma peripheral blood; left atrial blood)
- Year:
- 2024
- Journal Name:
- BMC Cardiovascular Disorders
- PubMed ID:
- 39261826
Biomimetic mucin modified PLGA nanoparticles for enhanced blood compatibility.
- Species:
- Human
- Sample Type:
- Platelet Rich Plasma
- Year:
- 2013
- Journal Name:
- Journal of Colloid and Interface Science
- PubMed ID:
- 23978287
Glucosylated polymeric nanoparticles: A sweetened approach against blood compatibility paradox
- Species:
- Human
- Sample Type:
- Plasma (platelet rich plasma)
- Year:
- 2013
- Journal Name:
- Colloids and Surfaces B: Biointerfaces
- PubMed ID:
- 23563302
Evaluation of Platelet Activation During an Asthmatic Attack in Children
- Species:
- Human
- Sample Type:
- Serum (Asthamtic attack patients)
- Year:
- 2015
- Journal Name:
- Turkish Journal of Pediatric Disease
Impaired transport function of the left atrium and left atrial appendage in cryptogenic stroke patients with atrial septal aneurysm and without patent foramen ovale
- Species:
- Human
- Year:
- 2011
- Journal Name:
- European Journal of Echocardiography
- PubMed ID:
- 21112956
Improved endothelial function in patients with atrial fibrillation through maintenance of sinus rhythm by successful catheter ablation.
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2011
- Journal Name:
- Journal of Cardiovascular Electrophysiology
- PubMed ID:
- 20958832
