Human Papillomavirus IgM antibody (HPV-Ab-IgM) ELISA Kit
Référence AE62502HU-480
Conditionnement : 5x96tests
Marque : Abebio
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Téléphone : +1 850 650 7790
Reagent Preparation
Results demonstration
Assay Procedure Summary
Certificate
Product Details
| Species Reactivity | Human (Homo sapiens) |
| UniProt | N/A |
| Abbreviation | HPV-Ab-IgM |
| Alternative Names | N/A |
| Range | Request Information |
| Sensitivity | Request Information |
| Sample Type | Serum, Plasma, Other biological fluids |
| Detection Method | Indirect ELISA |
| Analysis Method | Qualitative |
| Assay Duration | 1-3h |
| Sample Volume | 1-200 μL |
| Detection Wavelengt | 450 nm |
Test principle
The microtiter plate provided in this kit has been pre-coated with HPV-Ag. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated HPV-Ab-IgM and incubated. Then substrate solutions are added to each well. Only those wells that contain HPV-Ab-IgM and enzyme-conjugated HPV-Ab-IgM will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.
Product Overview
Papillomaviridae is an ancient taxonomic family of non-enveloped DNA viruses, collectively known as papillomaviruses. Several hundred species of papillomaviruses, traditionally referred to as "types", have been identified infecting all carefully inspected mammals, but also other amniotes such as birds, snakes and turtles. Infection by most papillomavirus types, depending on the type, is either asymptomatic (e.g. most Beta-PVs) or causes small benign tumors, known as papillomas or warts (e.g. human papillomavirus1, HPV6 or HPV11). Papillomas caused by some types, however, such as human papillomaviruses 16 and 18, carry a risk of becoming cancerous.
Components
Reagents | Quantity | Reagents | Quantity |
Assay plate (96 Wells) | 1 | Instruction manual | 1 |
Positive Control | 1 x 1 mL | Negative Control | 1 x 1 mL |
Sample Diluent | 1 x 20 mL | HRP-Conjugate | 1 x 12 mL |
Wash Buffer (concentrate 25 x) | 1 x 20 mL | Stop Solution | 1 x 6 mL |
Substrate A | 1 x 6 mL | Substrate B | 1 x 6 mL |
Adhesive Films | 4 |
Specificity
This assay has high sensitivity and excellent specificity for detection of HPV-Ab-IgM. No significant cross-reactivity or interference between HPV-Ab-IgM and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant HPV-Ab-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of HPV-Ab-IgM in samples.
Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HPV-Ab-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Stability
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
Sample collection and storage
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Kits storage instructions
Store at 2-8°C. Please refer to Instruction Manual.