Human PGLYRP1 (Peptidoglycan recognition protein 1) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	PGLYRP1
Gene ID:	8993
Uniprot ID:	PGRP1_HUMAN
Specificity:	This kit recognizes Human PGRP-S in samples. No significant cross-reactivity or interference between Human PGRP-S and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	N-glycosylated. N-glycosylation is required for bactericidal activity.
Function	Innate immunity protein that plays several important functions in antimicrobial and antitumor defense systems. Acts as a pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria and thus provides bactericidal activity. Forms an equimolar complex with heat shock protein HSPA1A and induces programmed cell death through apoptosis and necroptosis in tumor cell lines by activating the TNFR1 receptor on the target cell membrane. In addition, acts in complex with the Ca(2+)-binding protein S100A4 as a chemoattractant able to induce lymphocyte movement. Mechanistically, this complex acts as a ligand of the chemotactic receptors CCR5 and CXCR3 which are present on the cells of the immune system. Promotes also the activation of lymphocytes that become able to kill virus-infected cells as well as tumor cells by modulating the spectrum of their target-cell specificity. Induction of cytotoxicity on monocyte surface requires interaction with TREM1 receptor.
Protein Name	Peptidoglycan Recognition Protein 1 Peptidoglycan Recognition Protein Short Pgrp-S
Database Links	Reactome: R-HSA-6798695 Reactome: R-HSA-6803157
Cellular Localisation	Secreted Cytoplasmic Granule
Alternative ELISA Names	Peptidoglycan Recognition Protein 1 ELISA kit Peptidoglycan Recognition Protein Short ELISA kit Pgrp-S ELISA kit PGLYRP1 ELISA kit PGLYRP ELISA kit PGRP ELISA kit TNFSF3L ELISA kit SBBI68 ELISA kit UNQ639 ELISA kit PRO1269 ELISA kit
Specificity	This kit recognizes Human PGRP-S in samples. No significant cross-reactivity or interference between Human PGRP-S and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-110	100
EDTA plasma (n=8)	83-97	90
Cell culture media (n=8)	86-99	92

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.93	2.75	8.01	1.01	2.90	8.66
Standard deviation	0.05	0.16	0.27	0.05	0.16	0.47
CV (%)	5.38	5.82	3.37	4.95	5.52	5.43

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance