Human TGM2 (Protein-glutamine gamma-glutamyltransferase 2) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	TGM2
Gene ID:	7052
Uniprot ID:	TGM2_HUMAN
Specificity:	This kit recognizes Human TGM2 in samples. No significant cross-reactivity or interference between Human TGM2 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Disulfide bond formation inactivates the calcium-dependent acyltransferase activity. Cys-370 can form disulfide bonds with both Cys-230 and Cys-371: formation of a disulfide bond between Cys-230 and Cys-370 facilitates formation of the disulfide between Cys-370 and Cys-371, which promotes inactivation of the acyltransferase activity. May also form interchain disulfids between Cys-230 and Cys-370. Ca(2+) protects against disulfide bond formation and inactivation. Auto-transglutaminated: Forms covalent cross-links mediated by transglutaminase between Gln-633 and the epsilon-amino group of a lysine residue of itself or HMGB1, forming homopolymers and heteropolymers, respectively. S-nitrosylated, leading to inactivation of the acyltransferase activity.
Function	Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis. Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP.inhibition is relieved by Ca(2+) in response to various stresses. When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds. Plays a key role during apoptosis, both by (1) promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm, and by (2) mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components. In addition to protein cross-linking, can use different monoamine substrates to catalyze a vast array of protein post-translational modifications: mediates aminylation of serotonin, dopamine, noradrenaline or histamine into glutamine residues of target proteins to generate protein serotonylation, dopaminylation, noradrenalinylation or histaminylation, respectively. Mediates protein serotonylation of small GTPases during activation and aggregation of platelets, leading to constitutive activation of these GTPases. Plays a key role in chromatin organization by mediating serotonylation and dopaminylation of histone H3. Catalyzes serotonylation of 'Gln-5' of histone H3 (H3Q5ser) during serotonergic neuron differentiation, thereby facilitating transcription. Acts as a mediator of neurotransmission-independent role of nuclear dopamine in ventral tegmental area (VTA) neurons: catalyzes dopaminylation of 'Gln-5' of histone H3 (H3Q5dop), thereby regulating relapse-related transcriptional plasticity in the reward system. Regulates vein remodeling by mediating serotonylation and subsequent inactivation of ATP2A2/SERCA2. Also acts as a protein deamidase by mediating the side chain deamidation of specific glutamine residues of proteins to glutamate. Catalyzes specific deamidation of protein gliadin, a component of wheat gluten in the diet. May also act as an isopeptidase cleaving the previously formed cross-links. Also able to participate in signaling pathways independently of its acyltransferase activity: acts as a signal transducer in alpha-1 adrenergic receptor-mediated stimulation of phospholipase C-delta (PLCD) activity and is required for coupling alpha-1 adrenergic agonists to the stimulation of phosphoinositide lipid metabolism. Isoform 2: Has cytotoxic activity: is able to induce apoptosis independently of its acyltransferase activity.
Protein Name	Protein-Glutamine Gamma-Glutamyltransferase 2 Erythrocyte Transglutaminase Heart G Alpha(H Hhg Alpha(H Isopeptidase Tgm2 Protein G Alpha(H G(H Protein-Glutamine Deamidase Tgm2 Protein-Glutamine Dopaminyltransferase Tgm2 Protein-Glutamine Histaminyltransferase Tgm2 Protein-Glutamine Noradrenalinyltransferase Tgm2 Protein-Glutamine Serotonyltransferase Tgm2 Tissue Transglutaminase Ttg Ttgase Transglutaminase C Tg(C Tgc Tgase C Transglutaminase H Tgase H Transglutaminase Ii Tgase Ii Transglutaminase-2 Tg2 Tgase-2 Htg2
Cellular Localisation	Cytoplasm Cytosol Nucleus Chromosome Secreted Extracellular Space Extracellular Matrix Cell Membrane Mitochondrion Mainly Localizes To The Cytosol Present At Much Lower Level In The Nucleus And Chromatin Also Secreted Via A Non-Classical Secretion Pathway To The Extracellular Matrix Isoform 2: Cytoplasm Perinuclear Region
Alternative CLIA Names	Protein-Glutamine Gamma-Glutamyltransferase 2 CLIA kit Erythrocyte Transglutaminase CLIA kit Heart G Alpha(H CLIA kit Hhg Alpha(H CLIA kit Isopeptidase Tgm2 CLIA kit Protein G Alpha(H CLIA kit G(H CLIA kit Protein-Glutamine Deamidase Tgm2 CLIA kit Protein-Glutamine Dopaminyltransferase Tgm2 CLIA kit Protein-Glutamine Histaminyltransferase Tgm2 CLIA kit Protein-Glutamine Noradrenalinyltransferase Tgm2 CLIA kit Protein-Glutamine Serotonyltransferase Tgm2 CLIA kit Tissue Transglutaminase CLIA kit Ttg CLIA kit Ttgase CLIA kit Transglutaminase C CLIA kit Tg(C CLIA kit Tgc CLIA kit Tgase C CLIA kit Transglutaminase H CLIA kit Tgase H CLIA kit Transglutaminase Ii CLIA kit Tgase Ii CLIA kit Transglutaminase-2 CLIA kit Tg2 CLIA kit Tgase-2 CLIA kit Htg2 CLIA kit TGM2 CLIA kit
Specificity	This kit recognizes Human TGM2 in samples. No significant cross-reactivity or interference between Human TGM2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-106	99
EDTA plasma(n=8)	97-110	104
Cell culture media(n=8)	93-108	99

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	93.24	199.01	925.97	100.67	188.15	1012.30
Standard deviation	12.06	20.42	61.95	9.80	16.03	74.40
CV (%)	12.93	10.26	6.69	9.73	8.52	7.35

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance