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> IgD (MaxLight 550)

IgD (MaxLight 550)

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Référence I1895-02A-ML550-100ul

Conditionnement : 100ul

Marque : US Biological

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I1895-02A-ML550 IgD (MaxLight 550)

Clone Type
Monoclonal
Host
mouse
Source
rat
Swiss Prot
P01883
Isotype
IgG1
Grade
Affinity Purified
Applications
FLISA IHC
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications:
Suitable for use in FLISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2268 (MARD-3)|Host: mouse|Source: rat|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.|Purity: Purified by affinity chromatography from tissue culture spernatant.|Immunogen: IR731 myeloma protein.|Specificity: Recognizes rat IgD and does not crossreact with other rat immunoglobulin classes. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
IR731 myeloma protein.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by affinity chromatography from tissue culture spernatant.
Specificity
Recognizes rat IgD and does not crossreact with other rat immunoglobulin classes.
References
. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat. J. Immunol. 121: 2077-2082. 2. Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunol. 4: 44-48. 3. Westermann, J. et al. (2005) Naive, effector, and memory T lymphocytes efficiently scan dendritic cells in vivo: contact frequency in T cell zones of secondary lymphoid organs does not depend on LFA-1 expression and facilitates survival of effector T cells. J Immunol. 174: 2517-24. 4. FrancoSalinas, G. et al. (2011) TNF blockade abrogates the induction of T cell-dependent humoral responses in an allotransplantation model J Leukoc Biol. 90: 367-75. 5. Zonneveld-Huijssoon, E. et al. (2011) Bystander suppression of experimental arthritis by nasal administration of a heat shock protein peptide. Ann Rheum Dis. 70: 2199-206.