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> IgE (HRP)

IgE (HRP)

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Référence I1900-30A-100ug

Conditionnement : 100ug

Marque : US Biological

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I1900-30A IgE (HRP)

Clone Type
Monoclonal
Host
mouse
Source
rat
Isotype
IgG1
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Affinity Constant:
4x10e9M-1

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2277 (MARE-1)|Host: mouse|Source: rat|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, 0.01% thimerosal, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).|Purity: Purified by affinity chromatography from tissue culture supernatent|Immunogen: Rat IgE myeloma protein (IR162, IR1016, IR2 ,IR410)|Specificity: Recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IgE myeloma protein (IR162, IR1016, IR2 ,IR410)
Form
Supplied as a liquid in PBS, 0.01% thimerosal, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Purity
Purified by affinity chromatography from tissue culture supernatent
Specificity
Recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.
References
1. Negrao-Cooea, D. et al. (1996) Intestinal transport and catabolism of IgE: a major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection of rats. J. Immunol. 157: 4037-4044. 2. Bazin, H.et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours. Immunology 26: 713-723. 3. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat. J. Immunol. 121: 2077-2082. 4. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum. J. Immunol. Methods. 71: 9-16. 5. Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants. J. Imm. Methods 66: 261-269.