Flow cytometry: 1-2 µg/ml. Immunoprecipitation. Western blot (non reducing conditions).
Reactivity
Human, Mouse, Primate
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Recombinant Vaccinia virus encoding CD222
Specificity
The antibody recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.
Buffer
PBS, pH~7.4 containing 15 mM Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
CD222 is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-b, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
Synonyms
CI-MPR, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, CI Man-6-P receptor, M6PR, Late Endosome Marker, M6P/IGF2R, MPR 300
Reference Data
Protein Categories
Cancer: Gastrointestinal, CD Markers, Growth Factors, Intracellular Proteins, Membrane Proteins
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