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> IgG, F(ab')2 (FITC)

IgG, F(ab')2 (FITC)

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Référence I1904-59U-500ug

Conditionnement : 500ug

Marque : US Biological

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I1904-59U IgG, F(ab')2 (FITC)

Clone Type
Polyclonal
Host
rabbit
Source
sheep
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IF WB
Crossreactivity
Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in Immunofluorescence and FLISA. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:1000-1:5000
FLISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.

Excitation Wavelength:
495nm

Emmission Wavelength:
528nm

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: sheep|Concentration: ~1.5mg/ml (UV280)|Form: Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC).. F/P Ratio: 2.8 moles FITC/mole Rabbit IgG|Purity: Purified by immunoaffinity chromatography. F/P Ratio: 2.8 moles DTAF/mole Rabbit IgG|Immunogen: Sheep IgG F(ab’)2 fragment.|Specificity: Recognizes sheep IgG, F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-rabbit serum, sheep IgG, sheep IgG F(ab’)2 and sheep serum. No reaction was observed against sheep IgG F(c).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Sheep IgG F(ab’)2 fragment.
Form
Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC).. F/P Ratio: 2.8 moles FITC/mole Rabbit IgG
Purity
Purified by immunoaffinity chromatography. F/P Ratio: 2.8 moles DTAF/mole Rabbit IgG
Specificity
Recognizes sheep IgG, F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-rabbit serum, sheep IgG, sheep IgG F(ab’)2 and sheep serum. No reaction was observed against sheep IgG F(c).