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> IgG (Fab)

IgG (Fab)

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Référence 045857-100ug

Conditionnement : 100ug

Marque : US Biological

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045857IgG (Fab)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcehuman
IsotypeIgG2b
GradeAffinity Purified
ApplicationsE FC WB
CrossreactivityHu
Shipping TempBlue Ice
Storage Temp-20°C

Immunoglobulin G (IgG) is a 150kD soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.

Applications:
Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2b|Clone No: 4A11|Host: mouse|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Purified human IgG.|Specificity: Recognizes Fab fragment of human IgG. Detects Fab fragments regardless light chain type.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified human IgG.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Fab fragment of human IgG. Detects Fab fragments regardless light chain type.
References
1. Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.|2. Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.|3. Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.|4. Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.