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> IgG2b (FITC)

IgG2b (FITC)

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Référence I1904-84E-1mg

Conditionnement : 1mg

Marque : US Biological

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I1904-84E IgG2b (FITC)

Clone Type
Polyclonal
Host
rabbit
Source
mouse
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IF WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in FLISA, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:500-1:2500
Optimal working dilutions to be determined by researcher.

Label: Fluorescein isothiocyanate (FITC)
Molecular Weight: 390D
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 2.4 moles FITC per mole of Rabbit IgG

Storage and Stability:
Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1mg/ml (after reconstitution) |Form: Supplied as a lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute in sterile 40-50% glycerol, dH2O.|Purity: Prepared by immunoaffinity chromatography.|Immunogen: Mouse IgG2b.|Specificity: Recognizes mouse IgG2b, g2b chain. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgG2b.
Form
Supplied as a lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute in 1ml sterile dH2O.
Purity
Prepared by immunoaffinity chromatography.
Specificity
Recognizes mouse IgG2b, g2b chain. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.