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> IgG2b (HRP)

IgG2b (HRP)

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Référence I1904-84H-HRP-100ul

Conditionnement : 100ul

Marque : US Biological

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I1904-84H-HRP IgG2b (HRP)

Clone Type
Monoclonal
Host
mouse
Source
rat
Isotype
IgG1
Grade
Affinity Purified
Applications
E
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
ELISA: 5ug/ml (as a coating antibody)
Optimal working dilutions to be determined by researcher.

Avidity for rat IgG2b: 4.7 x 10e9 M-1.

Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: MARG 2b-8|Host: mouse|Source: rat|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by affinity chromatography.|Immunogen: Rat IgG2b myeloma protein (IR863).|Specificity: Recognizes the rat IgG2b heavy chain. Does not crossreact with other immunoglobulin classes or subclasses.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat IgG2b myeloma protein (IR863).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by affinity chromatography.
Specificity
Recognizes the rat IgG2b heavy chain. Does not crossreact with other immunoglobulin classes or subclasses.
References
1. Sato, K., et al., Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat transplant. J. Immunol. 166: 4185-4194 (2001).