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IgM

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Référence I1905-15I-250ug

Conditionnement : 250ug

Marque : US Biological

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I1905-15I IgM

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P01871
Isotype
IgG1
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Ca Eq Fe Hu Mk Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1:10-1:50. Use 10ul to label 10e6 cells or 100ul whole blood. May be used as a marker of mature B lymphocytes.
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo
I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H2438 (CM7)|Host: mouse|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Purified human IgM, and then purified feline IgM.|Specificity: Recognizes human IgM. No crossreactivity with other immunoglobulin classes or sub classes. Species Crossreactivity: Rhesus and marmoset monkey, equine, feline, canine, porcine||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified human IgM, and then purified feline IgM.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human IgM. No crossreactivity with other immunoglobulin classes or sub classes. Species Crossreactivity: Rhesus and marmoset monkey, equine, feline, canine, porcine
References
1. Reubel, G.H. et al. (1992). Interaction of acute feline Herpes virus - 1 and chronic feline immunodeficiency virus infections in experimentally infected specific pathogen free cats. Vet. Immunol. Immunopathol. 35: 95-119. 2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 62 (2): 101-43. 3. Chandhasin, C. et al. (2005) Unique long terminal repeat and surface glycoprotein gene sequences of feline leukemia virus as determinants of disease outcome. J Virol. 79 (9): 5278-87. 4. Prieto, J.M. et al. (2017) Developmental expression of B cell molecules in equine lymphoid tissues. Vet Immunol Immunopathol. 183: 60-71. 5. Tallmadge, R.L. et al. (2012) Expression of essential B cell development genes in horses with common variable immunodeficiency Molecular Immunology. 51 (2): 169-76.