IgM (Immunoglobulin Mu Heavy Chain, AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region) (BSA & Azide Free) (MaxLight 750)
Référence MBS6236505-5x0,1mL
Conditionnement : 5x0.1mL
Marque : MyBiosource
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IgM Secondary Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6236505MBS6236505 is a secondary antibody targeting IgM. Also known as IgM (Immunoglobulin Mu Heavy Chain, AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region) (BSA & Azide Free) (MaxLight 750). Reactive in Human. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
IgM, Secondary Antibody
Full Product Name
IgM (Immunoglobulin Mu Heavy Chain, AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region) (BSA & Azide Free) (MaxLight 750)
Product Gene Name
IgM secondary antibody
Product Synonym Names
Immunoglobulin Mu Heavy Chain; AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
DA4-4; same as SA-DA4 or HB57
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human IgM (Immunoglobulin Mu Heavy Chain).
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Heavy chain of human IgM. Cellular Localization: Cytoplasm, Cell Surface and Secreted
Conjugate
MaxLight750
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen.
Product Categories/Family for IgM secondary antibody
Antibodies; Immunoglobulins
Applications Tested/Suitable for IgM secondary antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for IgM secondary antibody
FC/FACS: 0.5-1ug/million cells
FLISA: For coating, order Ab without BSA
WB: 0.5-1ug/ml
IF: 0.5-1ug/ml
IP: 0.5-1ug/500ug protein lysate
IHC-F/P: 0.5-1ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
FLISA: For coating, order Ab without BSA
WB: 0.5-1ug/ml
IF: 0.5-1ug/ml
IP: 0.5-1ug/500ug protein lysate
IHC-F/P: 0.5-1ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
Product References and Citations for IgM secondary antibody
1. Maruyama S, et. al. Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies. Journal of Immunology 1985; 135(1):192-9. 2. Rudich SM, et. al. Human B cell activation. Evidence for diverse signals provided by various monoclonal anti-IgM antibodies. Journal of Experimental Medicine, 1985; 162(4):1236-55.
Other Notes
Small volumes of IgM secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for IgM. It may not necessarily be applicable to this product.
NCBI GeneID
Protein Family
IgM protease
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.