Immunoplates for Tumor-derived Exosome capture and enrichment from human plasma
| Product: | Transparent 1 standard 96-well format pre-coated plate |
| Sample type: | Human plasma |
| Exosome capiture: | Enrichment in Tumor-derived exosome |
| Starting material: | Up to 100 µL of biological sample can be loaded per well. Unfractionated human plasma o sample can be directly used for capture. Concentrated urine samples are recommended prior capture according to our suggested protocol. |
| Product overview: | Our multiwell plates are standard 96-well format where assays can be conducted as singletons and/or multiple wells. This enables optimization of a wide range of sandwich ELISA assays or other downstream applications, such as RNA extraction and proteomic analyses. Transparent, white and black plates are available depending on the downstream detection approach (colorimetric, luminometric and fluorimetric). |
| Antibody used for coating: | Polyclonal rabbit antibody |
| Coating: | Covalent |
| Packaging: | The plate is packaged in an opaque aluminum pouch which complies to food and pharmaceutical regulations. Easy to open and re-sealable by zip-lock feature. |
| Shipment and storage: | Immunoplates are shipped at 4°C and storage conditions as recommended in the product insert. |
| Expiration date: | Unopened: 2 year stored at 4°C Opened: 1 month stored at 4°C |
Applications:
Proteomic analyses
Exosomes incorporate a wide range of membranes and cytosolic proteins involved in many cellular functions and reflecting both their endosomal origin and a protein fingerprint unique for parent cell type and condition. HBM Immunoplates are an useful platform for Exosome capture from different biological sources and allow quantification through ELISA technique of protein exosomal markers.
Work with exosomal RNA
Exosomes shuttle RNA molecules (mRNAs and miRNAs), both classes proved to be functional in target cell, being translated or modulating gene silencing, respectively. Overall, exosome molecular composition distinguishes resting from activated, healthy from diseased cells.
RNA extraction could be performed after capturing exosomes on the plate. Recommended for RNA extraction: HansaBioMed Exosome Total RNA Extraction Kit.
Total exosome quantification
Immunoplates are part of the Ready to Use Kit for capture and quantification. The kit contains all the necessary components exosome quantification.