• SKU SCR-038449
    Article Group

    Mounting Medium (Fluorescence)
    Product Name

    Immunoselect Antifading Mounting Medium PI
    Description

    contains nuclear staining dye propidium iodide, fluorescence mounting medium, no background autofluorescence, slowly hardening over weeks, strong reduction of photobleaching, viscous fluid, water-based
    Temperature - Storage

    2-8°C
    Search Code

    038449, 38449, SCR-38449
    Manufacturer / Brand

    dianova

  • Background:

    ImmunoSelect® Antifading Mounting Medium is developed for fluorescence-microscopical use combining strong initial fluorescence with drastical reduction of photobleaching over time. In contrast to several media ImmunoSelect® Antifading Mounting Medium does not exhibit autofluorescence at any common wavelength.

    ImmunoSelect® Antifading Mounting Medium is an aqueous mountant which does not solidify directly, but remains a viscous liquid for several days on the slide. To mount cellular material on the slide one drop of medium is sufficient. After a cover slip is applied, the medium disperses over the entire section. When mounted slides are stored at 4°C in the dark, fluorescence will remain for many weeks.

    Antifading Mounting Medium for multiple labeling

    Immunostaining of DNA adducts (red) and CNPase (green) in cryosections of the inner ear (stria vascularis) from mice (24 h after exposure to a DNA-reative platinum compound)*.

    Compatibility with Fluorochromes

    The Mounting Medium is tested for several fluorescence dyes:
    • Fluorescein derivatives, eg. FITC
    • Rhodamine derivatives, eg. Rhodamin Red-X, TRITC, Texas Red
    • All common fluorescent dyes, eg. Alexa Fluor dyes, DyLight dyes, Cyanine dyes
    • Phycobilliproteins, eg. PE
    • DAPI
    • Hoechst 33358 and 3334
    • Propidium Iodide

    * J. Thomale, Institute for Cell Biology, University of Duisburg-Essen, Germany