Insulin, Recombinant, Mouse (INS)

Recombinant protein corresponding to aa25-54 of mouse Insulin-B linked by polypeptide linker (GGGGS) to aa90-110 of mouse Insulin A with a N-terminal His-Tag, expressed in E. coli. UniProt/Accession: P01326.

Amino Acid Sequence:
FVKQHL CGSHLVEALY LVCGERGFFY TPMSGGGGSG IVDQCCTSIC SLYQLENYCN

Predicted Molecular Weight:
10.3kD

Accurate Molecular Weight:
10kD (SDS-PAGE reducing conditions)

Predicted Isoelectric Point:
7.1

Subcellular Location:
Secreted

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.