Interferon beta, IFN-beta/IFNB BioAssay™ ELISA Kit (Chicken)

For the quantitative determination of chicken Interferon beta in
serum, plasma, and tissue homogenates.

Sensitivity:
3.125pg/ml

Detection Range:
2.5-200pg/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of chicken IFN-beta. No significant cross-reactivity or interference between chicken IFN-beta and analogues was observed.

Assay Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IFN-beta and Horseradish Peroxidase (HRP) conjugated IFN-beta. The competitive inhibition reaction is launched between with HRP labeled IFN-beta and unlabeled IFN-beta with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IFN-beta in the sample. The color development is stopped and the intensity of the color is measured.

Kit Components:
Microtiter Strips, 1x96 wells
Standard, 6x0.5ml
HRP-conjugate, 1x6ml
Antibody, 1x6ml
Wash Buffer (20X), 1x15ml
Substrate A, 1x7ml
Substrate B, 1x7ml

Storage and Stability:
Store all kit components at 4°C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.