Interleukin 1 beta (IL-1b)

Interleukin-1 beta (IL-1beta) is a potent immunomodulator which mediates a wide range of immune and inflammatory responses, including the activation of B and T cells. Mouse IL-1b is a 17.5kD protein containing 153aa residues.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:10. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by researcher.

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo
I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Recommended Pair (only for sandwich detection of bovine IL-1b):
Capture: I7663-11K
Detection: I7663-10E

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.