Interleukin 1beta, IL-1beta BioAssay™ ELISA Kit (Chicken)

This chicken IL1B ELISA kit was designed for the quantitative measurement of chicken IL1B protein in serum, plasma, tissue homogenates.

Sensitivity:
0.27pg/ml

Detection Range:
0.27-200pg/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of chicken IL-1beta. No significant cross-reactivity or interference between chicken IL-1beta and analogues was observed.

Assay Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IL-1beta. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody specific to IL-1beta. The competitive inhibition reaction is launched between with pre-coated IL-1beta and IL-1beta in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-1beta in the sample. The color development is stopped and the intensity of the color is measured.

Kit Components:
Microtiter Strips, 1x96 wells
Standard (Freeze dried), 2xvial
Biotin-antibody (100X), 1x120ul
HRP-avidin (100X), 1x120ul
Biotin-antibody Diluent, 1x15ml
HRP-avidin Diluent, 1x15ml
Sample Diluent, 1x50ml
Wash Buffer (25X), 1x20ml
TMB Substrate, 1x10ml
Stop Solution: 1x10ml

Storage and Stability:
Store all kit components at 4°C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.