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Product Description
This is a non-reactive mouse monoclonal IgG2a kappa antibody labeled with one of our bright and photostable CF® Dyes and other labels. This antibody may serve as a control for a mouse monoclonal IgG2a antibody under identical experimental conditions. Biotium also carries mouse monoclonal isotype control antibodies for IgG1 Kappa (IGG1/1331) and IgG2b Kappa (IGG1/1333).
- Isotype control for antibody-based experiments
- Available with 6 bright and photostable CF® Dyes and biotin
- Suitable for western, flow cytometry, ELISA, immunofluorescence, and immunohistology
CF® Dyes offer exceptional brightness and photostability; learn more about CF® Dyes.
Catalog number key for antibody number 1331B, Anti-Isotype Control (MG2a-53)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified,
BSA-free | N/A | N/A | N/A | |
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
Technology
Primary & Secondary Antibody Conjugates
Tech Tips
Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Troubleshooting Tips for Fluorescence Staining
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Tech Tip: Fluorescence Bleed-Through and Cross-Talk
Product Attributes
Antibody reactivity (target)
None
Antibody type
Isotype Control
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Species reactivity
None (isotype control)
Antibody application notes
For coating for ELISA, order Ab without BSA, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Documents, Protocols, SDS and COA
Datasheet
Datasheet Download
COARequest the Certificate of Analysis
Citations
Note: Note: Listed references are for this antibody clone sold by Biotium and other suppliers.
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