ITK siRNA (Mouse)
Référence MBS829420-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
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ITK siRNA – Tyrosine-Protein Kinase ITK/TSK Isoform 1 (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829420MBS829420 is an siRNA targeting ITK. Also known as ITK siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit ITK expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
ITK, siRNA
Full Product Name
ITK siRNA (Mouse)
Product Gene Name
ITK sirna
Product Synonym Names
EMT; TLK; TSK; Tyrosine-protein kinase ITK/TSK; IL-2-inducible T-cell kinase; Kinase EMT; Kinase TLK; T-cell-specific kinase
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
ITK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
72,292 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ITK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for ITK sirna
RNA Interference (RNAi)
Other Notes
Small volumes of ITK sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for ITK. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tyrosine-protein kinase ITK/TSK isoform 1
NCBI Official Synonym Full Names
IL2 inducible T cell kinase
NCBI Official Symbol
Itk
NCBI Official Synonym Symbols
Emt; Tsk; Tcsk
UniProt Protein Name
Tyrosine-protein kinase ITK/TSK
UniProt Synonym Protein Names
IL-2-inducible T-cell kinase; Kinase EMT; Kinase TLK; T-cell-specific kinase
Protein Family
Tyrosine-protein kinase
UniProt Gene Name
Itk
UniProt Synonym Gene Names
Emt; Tlk; Tsk
UniProt Entry Name
ITK_MOUSE
NCBI Protein Information
tyrosine-protein kinase ITK/TSK
UniProt Comments for ITK
ITK: an intracellular tyrosine kinase of the Tec family expressed in T-cells. The protein contains both SH2 and SH3 domains and plays a role in T-cell proliferation and differentiation. Itk-deficient murine T cells display defects in TCR/CD3-induced actin polymerization.
Protein type: Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Tec family
Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; intercellular junction
Molecular Function: transferase activity; protein binding; metal ion binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; protein kinase activity
Biological Process: interleukin-4 production; adaptive immune response; peptidyl-tyrosine phosphorylation; immune system process; phospholipase C activation; interferon-gamma production; innate immune response; cytokine production; NK T cell differentiation; phosphorylation; protein amino acid phosphorylation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation
Show more Protein type: Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Tec family
Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; intercellular junction
Molecular Function: transferase activity; protein binding; metal ion binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; protein kinase activity
Biological Process: interleukin-4 production; adaptive immune response; peptidyl-tyrosine phosphorylation; immune system process; phospholipase C activation; interferon-gamma production; innate immune response; cytokine production; NK T cell differentiation; phosphorylation; protein amino acid phosphorylation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with ITK sirna
| Reference Product | PubMed Publications |
|---|---|
| Lck sirna | >60 publications with ITK and Lck |
| Lat sirna | >27 publications with ITK and Lat |
| Vav1 sirna | >19 publications with ITK and Vav1 |
| Grb2 sirna | >13 publications with ITK and Grb2 |
| Cd3d sirna | >3 publications with ITK and Cd3d |
| Cd247 sirna | >2 publications with ITK and Cd247 |
| Grap2 sirna | >2 publications with ITK and Grap2 |
| Plcg1 sirna | >2 publications with ITK and Plcg1 |
| Cd3g sirna | >2 publications with ITK and Cd3g |
| Lcp2 sirna | >1 publications with ITK and Lcp2 |
Pathways associated with ITK sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Adaptive Immune System Pathway antibodies | Adaptive Immune System Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| Chemokine Signaling Pathway antibodies | Chemokine Signaling Pathway Diagram |
| Chemokine Signaling Pathway antibodies | Chemokine Signaling Pathway Diagram |
| Chemokine Signaling Pathway antibodies | Chemokine Signaling Pathway Diagram |
| FCERI Mediated Ca+2 Mobilization Pathway antibodies | FCERI Mediated Ca+2 Mobilization Pathway Diagram |
| Fc Epsilon Receptor (FCERI) Signaling Pathway antibodies | Fc Epsilon Receptor (FCERI) Signaling Pathway Diagram |
| Generation Of Second Messenger Molecules Pathway antibodies | Generation Of Second Messenger Molecules Pathway Diagram |
| Immune System Pathway antibodies | Immune System Pathway Diagram |
| Innate Immune System Pathway antibodies | Innate Immune System Pathway Diagram |
Diseases associated with ITK sirna
| Disease Name | Pubmed Publications |
|---|---|
| Inflammation Antibodies | >39 publications with ITK and Inflammation |
| Lung Diseases Antibodies | >26 publications with ITK and Lung Diseases |
| Disease Models, Animal Antibodies | >21 publications with ITK and Disease Models, Animal |
| Neoplasms, Experimental Antibodies | >11 publications with ITK and Neoplasms, Experimental |
| Heart Diseases Antibodies | >7 publications with ITK and Heart Diseases |
| Bronchial Hyperreactivity Antibodies | >6 publications with ITK and Bronchial Hyperreactivity |
| Congenital Abnormalities Antibodies | >5 publications with ITK and Congenital Abnormalities |
| Necrosis Antibodies | >4 publications with ITK and Necrosis |
| Hyperplasia Antibodies | >4 publications with ITK and Hyperplasia |
| Vascular Diseases Antibodies | >4 publications with ITK and Vascular Diseases |
Organs/Tissues associated with ITK sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >257 publications with ITK and Blood |
| Thymus Antibodies | >39 publications with ITK and Thymus |
| Spleen Antibodies | >22 publications with ITK and Spleen |
| Bone Marrow Antibodies | >19 publications with ITK and Bone Marrow |
| Skin Antibodies | >12 publications with ITK and Skin |
| Lymph Node Antibodies | >11 publications with ITK and Lymph Node |
| Heart Antibodies | >10 publications with ITK and Heart |
| Kidney Antibodies | >5 publications with ITK and Kidney |
| Embryonic Tissue Antibodies | >3 publications with ITK and Embryonic Tissue |