JUN siRNA (Mouse)
Référence MBS829472-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
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JUN siRNA – Transcription Factor Ap-1 (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829472MBS829472 is an siRNA targeting JUN. Also known as JUN siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit JUN expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
JUN, siRNA
Full Product Name
JUN siRNA (Mouse)
Product Gene Name
JUN sirna
Product Synonym Names
Transcription factor AP-1; AH119; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; Jun A
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
JUN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
35,944 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse JUN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for JUN sirna
RNA Interference (RNAi)
Other Notes
Small volumes of JUN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for JUN. It may not necessarily be applicable to this product.
NCBI Official Full Name
transcription factor AP-1
NCBI Official Synonym Full Names
jun proto-oncogene
NCBI Official Symbol
Jun
NCBI Official Synonym Symbols
AP-1; Junc; c-jun
UniProt Protein Name
Transcription factor AP-1
UniProt Synonym Protein Names
AH119; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; Jun A
Protein Family
Transcription factor
UniProt Gene Name
Jun
UniProt Synonym Gene Names
AP1; Jun A
UniProt Entry Name
JUN_MOUSE
NCBI Protein Information
transcription factor AP-1
UniProt Comments for JUN
Jun: the protooncogene c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The Jun Kinases (JNKs) binds to the N-terminal region of c-Jun and phosphorylate it in response to extracellular signals including growth factors, cytokines and stress, regulating its transcriptional activity.
Protein type: Oncoprotein; Transcription factor
Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; nuclear chromatin; cytosol; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; enzyme binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; positive regulation of monocyte differentiation; positive regulation of apoptosis; regulation of cell cycle; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; microglial cell activation; SMAD protein nuclear translocation; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; response to radiation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of neuron apoptosis; positive regulation of cell proliferation; negative regulation of protein amino acid autophosphorylation; angiogenesis; negative regulation of neuron apoptosis; response to drug; release of cytochrome c from mitochondria; transcription, DNA-dependent; monocyte differentiation; leading edge cell differentiation; axon regeneration; learning; liver development; negative regulation of DNA binding; membrane depolarization; cellular response to potassium ion starvation; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cellular process; positive regulation of DNA replication; negative regulation of apoptosis
Show more Protein type: Oncoprotein; Transcription factor
Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; nuclear chromatin; cytosol; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; enzyme binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; positive regulation of monocyte differentiation; positive regulation of apoptosis; regulation of cell cycle; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; microglial cell activation; SMAD protein nuclear translocation; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; response to radiation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of neuron apoptosis; positive regulation of cell proliferation; negative regulation of protein amino acid autophosphorylation; angiogenesis; negative regulation of neuron apoptosis; response to drug; release of cytochrome c from mitochondria; transcription, DNA-dependent; monocyte differentiation; leading edge cell differentiation; axon regeneration; learning; liver development; negative regulation of DNA binding; membrane depolarization; cellular response to potassium ion starvation; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cellular process; positive regulation of DNA replication; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with JUN sirna
| Reference Product | PubMed Publications |
|---|---|
| Fos sirna | >8219 publications with JUN and Fos |
| Mapk3 sirna | >1895 publications with JUN and Mapk3 |
| Mapk8 sirna | >844 publications with JUN and Mapk8 |
| Atf2 sirna | >410 publications with JUN and Atf2 |
| Fosb sirna | >334 publications with JUN and Fosb |
| Atf3 sirna | >214 publications with JUN and Atf3 |
| Mapk14 sirna | >85 publications with JUN and Mapk14 |
| Fosl1 sirna | >23 publications with JUN and Fosl1 |
| Mapk9 sirna | >12 publications with JUN and Mapk9 |
| Mapk10 sirna | >5 publications with JUN and Mapk10 |
Pathways associated with JUN sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Activated TLR4 Signalling Pathway antibodies | Activated TLR4 Signalling Pathway Diagram |
| Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies | Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram |
| Amphetamine Addiction Pathway antibodies | Amphetamine Addiction Pathway Diagram |
| Amphetamine Addiction Pathway antibodies | Amphetamine Addiction Pathway Diagram |
| Androgen Receptor Signaling Pathway antibodies | Androgen Receptor Signaling Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| Cellular Senescence Pathway antibodies | Cellular Senescence Pathway Diagram |
Diseases associated with JUN sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >186410 publications with JUN and Nervous System Diseases |
| Cardiovascular Diseases Antibodies | >169326 publications with JUN and Cardiovascular Diseases |
| Brain Diseases Antibodies | >89059 publications with JUN and Brain Diseases |
| Heart Diseases Antibodies | >83686 publications with JUN and Heart Diseases |
| Pain Antibodies | >56824 publications with JUN and Pain |
| Inflammation Antibodies | >44912 publications with JUN and Inflammation |
| Liver Diseases Antibodies | >44816 publications with JUN and Liver Diseases |
| Kidney Diseases Antibodies | >38217 publications with JUN and Kidney Diseases |
| Hypertension Antibodies | >35857 publications with JUN and Hypertension |
| Necrosis Antibodies | >29801 publications with JUN and Necrosis |
Organs/Tissues associated with JUN sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >131894 publications with JUN and Brain |
| Heart Antibodies | >103576 publications with JUN and Heart |
| Bone Antibodies | >90359 publications with JUN and Bone |
| Liver Antibodies | >81624 publications with JUN and Liver |
| Muscle Antibodies | >78778 publications with JUN and Muscle |
| Lung Antibodies | >63068 publications with JUN and Lung |
| Kidney Antibodies | >59898 publications with JUN and Kidney |
| Skin Antibodies | >57264 publications with JUN and Skin |
| Eye Antibodies | >43664 publications with JUN and Eye |
| Joint Antibodies | >34330 publications with JUN and Joint |