Kappa Chain (B Cells) (APC)

Référence MBS6122274-0,1mL

Conditionnement : 0.1mL

Marque : MyBiosource

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Kappa Chain (IGKC) Secondary Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6122274

MBS6122274 is a secondary antibody targeting IGKC. Also known as Kappa Chain (B Cells) (APC). Reactive in Human. Host: Mouse. More details

Product Overview

Product Name
Kappa Chain (IGKC), Secondary Antibody
Full Product Name
Kappa Chain (B Cells) (APC)
Product Gene Name
IGKC secondary antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
L1C1
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human kappa. Does not react with rat.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
Molecular Weight
~22.5kDa
3D Structure
ModBase 3D Structure for P01601
Immunogen
B lymphoma cells
Conjugate
APC
Positive Control
293 T, Raji or hPBL cells. Tonsil or Spleen
Special Handling
Light sensitive

Product Categories/Family for IGKC secondary antibody

Applications Tested/Suitable for IGKC secondary antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for IGKC secondary antibody
FC/FACS: 0.5-2ug/10e6 cells
IP: 1-2ug/500ug protein lysate
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of IGKC secondary antibody

Formalin-fixed, paraffin-embedded human tonsil stained with Kappa antibody (1:400) using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of B lymphocytes.
Mouse Anti-IGKC Monoclonal Secondary Antibody – Cat# MBS6122274

Product References and Citations for IGKC secondary antibody

1. Takahashi H et. al. Pathol Res Prac 189: 300-311 (1993). 2. Momose H et. al. Hum Pathol. 23:1115-1119 (1992).

Other Notes

Small volumes of IGKC secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IGKC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Ig kappa chain V-ID region 16
NCBI Official Synonym Full Names
immunoglobulin kappa constant
NCBI Official Symbol
IGKC
NCBI Official Synonym Symbols
Km; HCAK1; IGKCD
UniProt Protein Name
Ig kappa chain V-ID region 16
UniProt Synonym Protein Names
Ig kappa chain V-I region HK101
Protein Family
Ig kappa chain V region
UniProt Gene Name
IGKV1D-16
UniProt Entry Name
KV109_HUMAN

UniProt Comments for IGKC

KV109:

Protein type: Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: external side of plasma membrane; extracellular region; extracellular space; plasma membrane

Molecular Function: antigen binding; serine-type endopeptidase activity

Biological Process: B cell receptor signaling pathway; complement activation; complement activation, classical pathway; defense response to bacterium; innate immune response; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of B cell activation; receptor-mediated endocytosis; regulation of immune response; retinal homeostasis

Disease: Immunoglobulin Kappa Light Chain Deficiency
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with IGKC secondary antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 CD22 Mediated BCR Regulation Pathway antibodies  CD22 Mediated BCR Regulation Pathway Diagram
 Classical Antibody-mediated Complement Activation Pathway antibodies  Classical Antibody-mediated Complement Activation Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Creation Of C4 And C2 Activators Pathway antibodies  Creation Of C4 And C2 Activators Pathway Diagram
 FCERI Mediated Ca+2 Mobilization Pathway antibodies  FCERI Mediated Ca+2 Mobilization Pathway Diagram
 FCERI Mediated MAPK Activation Pathway antibodies  FCERI Mediated MAPK Activation Pathway Diagram
 FCERI Mediated NF-kB Activation Pathway antibodies  FCERI Mediated NF-kB Activation Pathway Diagram

Organs/Tissues associated with IGKC secondary antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11 publications with IGKC and Blood
 Lung Antibodies  >7 publications with IGKC and Lung
 Vascular Antibodies  >3 publications with IGKC and Vascular
 Muscle Antibodies  >2 publications with IGKC and Muscle
 Eye Antibodies  >2 publications with IGKC and Eye
 Uncharacterized Tissue Antibodies  >2 publications with IGKC and Uncharacterized Tissue
 Skin Antibodies  >1 publications with IGKC and Skin
 Intestine Antibodies  >1 publications with IGKC and Intestine