Keratan Sulfate
Référence MBS605265-0,1mg
Conditionnement : 0.1mg
Marque : MyBiosource
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Keratan Sulfate (CHST1) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS605265Aggrecan is the major proteoglycan of human articular cartilage. The core protein is substituted by a number of keratan sulfate and chondroitin sulfate glycosaminoglycan chains. Whereas chondroitin sulfate is widely distributed throughout the body, keratan… More details
Product Overview
Product Name
Keratan Sulfate (CHST1), Monoclonal Antibody
Full Product Name
Keratan Sulfate
Product Gene Name
anti-CHST1 antibody
Product Synonym Names
Anti -Keratan Sulfate
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4B3/D10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from 50mM Tris, pH 7.4. Reconstitute with 100ul sterile ddH2O.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Keratan-Sulfate. Preliminary results, based on western Blot of keratan sulfate from different sources, indicate specificity for keratan sulfate from articular cartilage. Minimal if any crossreactivity with keratan sulfate from intervertebral disc. This is in contrast to the epitope recognized by K0197, which recognizes a widely distributed keratan sulfate epitope. Well suited for detecting keratan sulfate fragments released during human inflammatory or degenerative joint diseases into synovial fluid and serum.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
46,715 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (45669239..45687206, complement). Location: 11p11.2
OMIM
603797
Immunogen
Purified human aggrecan
Product Categories/Family for anti-CHST1 antibody
Applications Tested/Suitable for anti-CHST1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CHST1 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: <1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 1ug/ml
Dilution: ELISA: <1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 1ug/ml
Other Notes
Small volumes of anti-CHST1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CHST1 purchase
MBS605265 is an antibody targeting CHST1. Also known as Keratan Sulfate. Reactive in Human. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for CHST1. It may not necessarily be applicable to this product.
NCBI Official Full Name
keratan sulfate Gal-6-sulfotransferase
NCBI Official Synonym Full Names
carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
NCBI Official Symbol
CHST1
NCBI Official Synonym Symbols
C6ST; KSST; GST-1; KS6ST; KSGal6ST
UniProt Protein Name
Carbohydrate sulfotransferase 1
UniProt Synonym Protein Names
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase
Protein Family
Carbohydrate sulfotransferase
UniProt Gene Name
CHST1
UniProt Synonym Gene Names
GST-1; KS6ST; KSGal6ST; KSST
UniProt Entry Name
CHST1_HUMAN
NCBI Protein Information
carbohydrate sulfotransferase 1; carbohydrate (chondroitin 6/keratan) sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
NCBI Summary for CHST1
This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]
UniProt Comments for CHST1
CHST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N- acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Protein type: Membrane protein, integral; EC 2.8.2.21; Transferase; Glycan Metabolism - keratan sulfate biosynthesis
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: Golgi membrane; integral to membrane
Molecular Function: sulfotransferase activity; keratan sulfotransferase activity
Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; galactose metabolic process; polysaccharide metabolic process; pathogenesis; inflammatory response
Show more Protein type: Membrane protein, integral; EC 2.8.2.21; Transferase; Glycan Metabolism - keratan sulfate biosynthesis
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: Golgi membrane; integral to membrane
Molecular Function: sulfotransferase activity; keratan sulfotransferase activity
Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; galactose metabolic process; polysaccharide metabolic process; pathogenesis; inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-CHST1 antibody
| Reference Product | PubMed Publications |
|---|---|
| CHST2 antibody | >4 publications with CHST1 and CHST2 |
| CHST5 antibody | >1 publications with CHST1 and CHST5 |
Pathways associated with anti-CHST1 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Disease Pathway antibodies | Disease Pathway Diagram |
| Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies | Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram |
| Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway antibodies | Glycosaminoglycan Biosynthesis - Keratan Sulfate Pathway Diagram |
| Glycosaminoglycan Metabolism Pathway antibodies | Glycosaminoglycan Metabolism Pathway Diagram |
| Keratan Sulfate Biosynthesis Pathway antibodies | Keratan Sulfate Biosynthesis Pathway Diagram |
| Keratan Sulfate/keratin Metabolism Pathway antibodies | Keratan Sulfate/keratin Metabolism Pathway Diagram |
| MPS I - Hurler Syndrome Pathway antibodies | MPS I - Hurler Syndrome Pathway Diagram |
| MPS II - Hunter Syndrome Pathway antibodies | MPS II - Hunter Syndrome Pathway Diagram |
| MPS IIIA - Sanfilippo Syndrome A Pathway antibodies | MPS IIIA - Sanfilippo Syndrome A Pathway Diagram |
| MPS IIIB - Sanfilippo Syndrome B Pathway antibodies | MPS IIIB - Sanfilippo Syndrome B Pathway Diagram |
Diseases associated with anti-CHST1 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Inflammation Antibodies | >2 publications with CHST1 and Inflammation |
| Ovarian Neoplasms Antibodies | >1 publications with CHST1 and Ovarian Neoplasms |
| Ovarian Diseases Antibodies | >1 publications with CHST1 and Ovarian Diseases |
Organs/Tissues associated with anti-CHST1 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Eye Antibodies | >1 publications with CHST1 and Eye |
| Lung Antibodies | >1 publications with CHST1 and Lung |
| Brain Antibodies | >1 publications with CHST1 and Brain |