LACBIDRAFT_192523 Antibody - HRP Conjugated
Référence OASA05814
Conditionnement : 0.1mg
Marque : Aviva Systems Biology
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LACBIDRAFT_192523 Antibody - HRP Conjugated (OASA05814)
| Datasheets/Manuals | Printable datasheet for LACBIDRAFT_192523 Antibody - HRP Conjugated (OASA05814) |
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| Predicted Species Reactivity | Yeast |
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| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | YL1/2 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | HRP |
| Application | ELISA|IHC-Fr|WB |
| Additional Information | Fusion Partners: Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line. |
| :: | Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Preservative Stabilisers: 0.01% Thiomersal HRP Stabiliser (BUF052A) |
| :: | Approx Protein Conc: IgG concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline pH7.4 |
| Reconstitution and Storage | 2°C to 8°C |
| Immunogen | Yeast tubulin. |
| Purification | Protein G Purified |
| Predicted Homology Based on Immunogen Sequence | Yeast |
| Concentration | 1 mg/ml |
| Specificity | TUBULIN ALPHA |
| Application Info | Western Blot: This product is suitable for use as a loading control. |
| Application Data | Application #1: HeLa whole cell lysate probed with Rat anti Tubulin Alpha:HRP  |
| Reference | 1. Kilmartin, J.V. et al. (1982) Rat monoclonal anti tubulin antibodies derived by using a new non secreting rat cell line. J. Cell Biol. 93: 576-582. 2. Wehland, J. et al. (1983) A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo J. Cell Biol. 97: 1467-1475. 3. Wallace, S.W. et al. (2010) Cdc42 Regulates Apical Junction Formation in Human Bronchial Epithelial Cells through PAK4 and Par6B. Mol Biol Cell. Jul 14. [Epub ahead of print] 4. Wehland, J. et al. (1984) Amino acid sequence requirements in the epitope recognized by the alpha tubulin specific rat monoclonal antibody YL1/2. EMBO. J. 3: 1295-1300. 5. Burns, R. (1987) Cytoskeleton. Tubulin's terminal tyrosine Nature 327: 103-104. 6. Skinner, R.H. et al. (1991) Use of the Glu-Glu-Phe C-terminal epitope for rapid purification of the catalytic domain of normal and mutant ras GTPase-activating proteins. J. Biol. Chem. 266: 14163-14166. |
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| Gene Symbol | LACBIDRAFT_192523 |
| Alias Symbols | SAA, PIG4, SAA2, TP53I4 |
| NCBI Gene Id | 6288 |
| Description of Target | RAT ANTI TUBULIN ALPHA:HRP |
| Uniprot ID | P0DJI8 |
| Protein Accession # | XP_001877048.1 |
| Protein Size (# AA) | 446 |
