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> M-CSF/CSF1 Protein, Human, Recombinant (E. coli)

M-CSF/CSF1 Protein, Human, Recombinant (E. coli)

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M-CSF/CSF1 Protein, Human, Recombinant (E. coli)

(Synonyms: MGI-IM, MGC31930, MCSF, Macrophage Colony Stimulating Factor, Lanimostim, CSF-1, CSF1, colony stimulating factor 1) Copy Product Info
M-CSF/CSF1 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P09603-3.
M-CSF/CSF1 Protein, Human, Recombinant (E. coli)
For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity
Bioactivity
ED 50 of 1.0-3.0 ng/ml, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 × 10 5 -1.0 × 10 6 units/mg.
Description
M-CSF/CSF1 Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is P09603-3.
Species
Human
Expression System
E. coli
TagTag Free
Accession NumberP09603-3
ConstructionGlu33-Ser190
Protein Purity
> 95% as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.
ReconstitutionIt is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS or Tris-HCl, pH 8.0 up to 100 μg/ml.
SynonymsMGI-IM, MGC31930, MCSF, Macrophage Colony Stimulating Factor, Lanimostim, CSF-1, CSF1, colony stimulating factor 1
Research Background
Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of of several diseases, including breast and endometrial cancers. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain, and it is only active in the disulfide-linked dimeric form, which is bonded at Cys63.
Chemical Properties
Molecular Weight~28 kDa (Non-reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageUpon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.