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> M-CSF/CSF1 Protein, Rat, Recombinant (E. coli)

M-CSF/CSF1 Protein, Rat, Recombinant (E. coli)

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Référence TMPS-00105-50ug

Conditionnement : 50ug

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M-CSF/CSF1 Protein, Rat, Recombinant (E. coli)

(Synonyms: MGI-IM, MGC31930, MCSF, Macrophage Colony Stimulating Factor, Lanimostim, CSF-1, CSF1, colony stimulating factor 1) Copy Product Info
M-CSF/CSF1 Protein, Rat, Recombinant (E. coli) is expressed in E. coli. The accession number is Q8JZQ0-1.
M-CSF/CSF1 Protein, Rat, Recombinant (E. coli)
For research use only—not for human use. No sales to individuals. Use as intended only.

Batch Information

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Purity:> 95% as analyzed by SDS-PAGE
Appearance:Lyophilized powder
Color:White

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COAUser Guide of Recombinant Proteins

Product Introduction

Bioactivity
Bioactivity
ED 50 < 5.0 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 2.0 × 10 5 units/mg.
Description
M-CSF/CSF1 Protein, Rat, Recombinant (E. coli) is expressed in E. coli. The accession number is Q8JZQ0-1.
Species
Rat
Expression System
E. coli
TagTag Free
Accession NumberQ8JZQ0-1
ConstructionGlu33-Pro186
Protein Purity
> 95% as determined by SDS-PAGE
Endotoxin< 0.2 EU/μg of protein as determined by the LAL method.
FormulationLyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsMGI-IM, MGC31930, MCSF, Macrophage Colony Stimulating Factor, Lanimostim, CSF-1, CSF1, colony stimulating factor 1
Research Background
Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of of several diseases, including breast and endometrial cancers. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain, and it is only active in the disulfide-linked dimeric form, which is bonded at Cys63.
Chemical Properties
Molecular Weight~28 kDa (Reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageUpon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.