Maackia amurensis (MAA) (Agarose)

Purified Maackia amurensis (MAA) covalently linked to agarose beads (exclusion limit MW 5-15 million). Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Bead Size:
50-250 microns

Spacer:
None

Linkage:
Imidoester

pH:
4-9

Agarose Beads:
Seprapore 4B

Ligand Density:
5mg MAA/ml

Concentration:
2-3mg purified MAA per 1ml settled beads.

Form:
Supplied as a suspension in PBS, pH 7.3, 0.05% sodium azide. Covalently linked to agarose beads (exclusion limit MW 5-15 million).

Carbohydrate for Elution:
Simple sugars are not effective for elution. It is recommended that either 0.1M glycine buffer, pH 3.5, unbuffered 20mM ethylenediamine or 20mM diaminopropane be used for elution, depending on the stability of the glycoprotein at different pH values.

Storage and Stability:
May be stored at 4°C. Do Not Freeze. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.