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> Major Capsid Protein, Recombinant, Giardia Lamblia Virus, aa1-206, His-Tag (Gag)

Major Capsid Protein, Recombinant, Giardia Lamblia Virus, aa1-206, His-Tag (Gag)

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Référence 374122-100ug

Conditionnement : 100ug

Marque : US Biological

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374122Major Capsid Protein, Recombinant, Giardia Lamblia Virus, aa1-206, His-Tag (Gag)

Specifications
Swiss ProtQ67652
GradePurified
Shipping TempBlue Ice
Storage Temp-20°C

Capsid protein self-assembles to form an icosahedral capsid with a T=2 symmetry, 35 nm in diameter, and consisting of 60 capsid proteins asymmetric dimers. The capsid encapsulates the genomic dsRNA and the polymerase and remains intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of the virion and are extruded into the cytoplasm (By similarity).

Source:
Recombinant protein corresponding to aa1-206 from giardia lamblia virus gag, fused to His-Tag at N-terminal expressed in E. coli.

Molecular Weight:
~26.8kD

Amino Acid Sequence:
MVWGTGYGNPSPLNPYGFASLHRGGLNPLILAPENITFDTLNTHNDHEETHGESPEVPKASIAPAGRQNVPVLQQNQENEDNHALGGSEDAKDEREIRFSAIKTLYNYYSEGPSTPIMPHLVNRLRGLDALAKIDATLTKVDMNAAYIFALRPTFPYSYGYKQRFSNRRLTTSALCYARTGLSSFLTVDKTYTSNSPLKGGSRGWP

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Source: Recombinant, E. coli|Purity: ≥90% (SDS-PAGE)|Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Purity
≥90% (SDS-PAGE)
References
1. "Giardiavirus double-stranded RNA genome encodes a capsid polypeptide and a gag-pol-like fusion protein by a translation frameshift." Wang A.L., Yang H.M., Shen K.A., Wang C.C.Proc. Natl. Acad. Sci. U.S.A. 90:8595-8599(1993).