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> MHC Class I Monomorphic (PE)

MHC Class I Monomorphic (PE)

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Référence 215007-PE-100ul

Conditionnement : 100ul

Marque : US Biological

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215007-PE MHC Class I Monomorphic (PE)

Clone Type
Monoclonal
Host
mouse
Source
bovine
Swiss Prot
Q30289
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC WB
Crossreactivity
Bo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Clone recognizes a monomorphic determinant within the heavy chain of bovine MHC Class I. Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This product will immunoprecipitate a band of ~45kD under reducing conditions.

Applications:
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: IL­A88|Host: mouse|Source: bovine|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Bovine peripheral blood mononuclear cells.|Specificity: Recognizes bovine MHC Class I Monomorphic.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Bovine peripheral blood mononuclear cells.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine MHC Class I Monomorphic.
References
1. Ambagala, A.P.N. et al. (2000) An early pseudorabies virus protein down-regulates porcine MHC Class I expression by inhibition of transporter associated with antigen processing (TAP).J. Immunol. 164: 93-99. 2. Daubenberger, C.A. et al. (1999) Bovine gamma delta T-cell responses to the intracellular protozoan parasite Theileria parva.Infect. Immun. 67: 2241-2249. 3. Araibi, E.H. et al. (2004) Downregulation of major histocompatibility complex class I in bovine papillomas.J. Gen. Virol. 85: 2809-2814. 4. Ashrafi, G.H. et al. (2002) Down-regulation of MHC class I by bovine papillomavirus E5 oncoproteins.Oncogene. 21: 248-259. 5. Suzuki, T. et al. (2003) Evaluation of the delta subunit of bovine adaptor protein complex 3 as a receptor for bovine leukemia virus.J. Gen. Virol. 84: 1309-1316. 6. Stephens, S.A. et al. (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.Parasitology. 124: 485-493. 7. Toye, P.G. et al. (1990) Transfection into mouse L cells of genes encoding two serologically and functionally distinct bovine class I MHC molecules from a MHC-homozygous animal: evidence for a second class I locus in cattle.Immunology. 70: 20-26. 8. Marchetti, B. et al. (2002) The bovine papillomavirus oncoprotein E5 retains MHC class I molecules in the Golgi apparatus and prevents their transport to the cell surface.Oncogene. 21:7808-16 9. Bainbridge, DR. et al. (2001) Increased expression of major histocompatibility complex (MHC) class I transplantation antigens in bovine trophoblast cells before fusion with maternal cells.Reproduction.122:907-13. 10. Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.Immunology. 108:55-61.