MitoSOX Red [1003197-00-9]
Référence HY-D1055-100ug
Conditionnement : 100ug
Marque : MedChemExpress
MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems. The oxidized MitoSOX Red then binds to nucleic acids in mitochondria/nucleus, producing strong red fluorescence. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. In addition, superoxide dismutase (SOD) can prevent the oxidation of MitoSOX Red.
Excitation/emission wavelength: 510/580 nm.
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MitoSOX Red Chemical Structure
CAS No. : 1003197-00-9
This product is a controlled substance and not for sale in your territory.
Based on 24 publication(s) in Google Scholar
Description |
MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems. The oxidized MitoSOX Red then binds to nucleic acids in mitochondria/nucleus, producing strong red fluorescence. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. In addition, superoxide dismutase (SOD) can prevent the oxidation of MitoSOX Red. |
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In Vitro |
General Protocol MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
759.70 |
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Formule |
C43H43IN3P |
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CAS No. |
1003197-00-9 |
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Appearance |
Solid |
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Color |
Yellow to brown |
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Emission (Em) |
580 |
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Excitation (Ex) |
510 |
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SMILES |
NC1=CC(C(C2=CC=CC=C2)N(CCCCCC[P+](C3=CC=CC=C3)(C4=CC=CC=C4)C5=CC=CC=C5)C6=CC(N)=CC=C76)=C7C=C1.[I-] |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
-20°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
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Solvant et solubilité |
In Vitro:
DMSO : 100 mg/mL (131.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation | |||||||||||||
Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 1.3163 mL | 6.5815 mL | 13.1631 mL | 32.9077 mL |
5 mM | 0.2633 mL | 1.3163 mL | 2.6326 mL | 6.5815 mL | |
10 mM | 0.1316 mL | 0.6582 mL | 1.3163 mL | 3.2908 mL | |
15 mM | 0.0878 mL | 0.4388 mL | 0.8775 mL | 2.1938 mL | |
20 mM | 0.0658 mL | 0.3291 mL | 0.6582 mL | 1.6454 mL | |
25 mM | 0.0527 mL | 0.2633 mL | 0.5265 mL | 1.3163 mL | |
30 mM | 0.0439 mL | 0.2194 mL | 0.4388 mL | 1.0969 mL | |
40 mM | 0.0329 mL | 0.1645 mL | 0.3291 mL | 0.8227 mL | |
50 mM | 0.0263 mL | 0.1316 mL | 0.2633 mL | 0.6582 mL | |
60 mM | 0.0219 mL | 0.1097 mL | 0.2194 mL | 0.5485 mL | |
80 mM | 0.0165 mL | 0.0823 mL | 0.1645 mL | 0.4113 mL | |
100 mM | 0.0132 mL | 0.0658 mL | 0.1316 mL | 0.3291 mL |
MitoSOX Red Related Classifications
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
MitoSOX Red1003197-00-9Fluorescent DyeReactive Oxygen SpeciesMitochondrial MetabolismMitochondrial ROS Indicatorfluorescent dyemitochondrial ROSInhibitorinhibitorinhibit