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> Monkey Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA Kit

Monkey Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA Kit

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Référence EK6266-96T

Conditionnement : 96T

Marque : Signalway Antibody

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Product Detail

Product NameMonkey Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA Kit

ApplicationsELISA

Species ReactivityMonkey (Simian)

Other NamesDADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein|TNF superfamily; member 2|TNF; macrophage-derived|TNF; monocyte-derived|cachectin|tumor necrosis factor alpha

StorageThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

Application Details
Detect Range:7.8-500 pg/mL
Sensitivity:2.7 pg/mL
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm
Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TNF/TNFA/TNFSF6 in samples. An antibody specific for TNF/TNFA/TNFSF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNF/TNFA/TNFSF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF/TNFA/TNFSF6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF/TNFA/TNFSF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.

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