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> mouse Anti-CD1a (CD1, CD1A Antigen, Cortical Thymocyte Antigen CD1A, Epidermal Dendritic Cell Marker CD1a, FCB 6, HTA1, T Cell Surface Antigen Leu 6, Cell Surface Antigen T6, T Cell Surface Antigen T6/Leu 6, T Cell Surface Glycoprotein CD1A, T6, Thymocyte Antigen CD1A) Monoclonal Antibody [Clone:3H643 (NA1/34-HLK)]

mouse Anti-CD1a (CD1, CD1A Antigen, Cortical Thymocyte Antigen CD1A, Epidermal Dendritic Cell Marker CD1a, FCB 6, HTA1, T Cell Surface Antigen Leu 6, Cell Surface Antigen T6, T Cell Surface Antigen T6/Leu 6, T Cell Surface Glycoprotein CD1A, T6, Thymocyte Antigen CD1A) Monoclonal Antibody [Clone:3H643 (NA1/34-HLK)]

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Référence C2252-01W-100ug

Conditionnement : 100ug

Marque : US Biological

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Téléphone : +1 850 650 7790

C2252-01W CD1a (CD1, CD1A Antigen, Cortical Thymocyte Antigen CD1A, Epidermal Dendritic Cell Marker CD1a, FCB 6, HTA1, T Cell Surface Antigen Leu 6, Cell Surface Antigen T6, T Cell Surface Antigen T6/Leu 6, T Cell Surface Glycoprotein CD1A, T6, Thymocyte Antigen CD1A)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P06126
Isotype
IgG2a
Grade
Highly Purified
Applications
FC IHC
Crossreactivity
Ca Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD1a is a ~49kDa single pass type 1 transmembrane glycoprotein containing a single Ig-like domain, expressed in association with beta 2 microglobulin. CD1a is expressed strongly by cortical thymocytes, and also by Langerhans cells and interdigitating cells. CD1a is involved in the presentation of lipids and glycolipids to NK cells.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1/Ag4.1 myeloma cells with spleen cells from Balb/c mice.

Positive Control for Immunohistochemistry:
Skin

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 3H643 (NA1/34-HLK)|Host: mouse|Source: human|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide|Purity: Purified by Ion Exchange chromatography|Immunogen: Human thymocytes|Specificity: Recognizes human CD1. Species Crossreactivity: canine and Cynomolgus monkey||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human thymocytes
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Purity
Purified by Ion Exchange chromatography
Specificity
Recognizes human CD1. Species Crossreactivity: canine and Cynomolgus monkey
References
1. 1. McMichael, A. J. et al. (1979) A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur. J. Immunol. 9: 205-210, 2. Poulter, L. W. et al. (1986) Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies. Scand. J. Immunol. 24: 351-357, 3. Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leucocytes. Vet. Immunol. Immunopathol. 53: 329-334, 4. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross reactive antibodies. Exp. Anim. 49: 97-110.