Principle of the Assay: dsDNA IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-dsDNA IgG antibody and an dsDNA IgG-HRP conjugate. The assay sample and buffer are incubated together with dsDNA IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the dsDNA IgG concentration since dsDNA IgG from samples and dsDNA IgG-HRP conjugate compete for the anti-dsDNA IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by dsDNA IgG from the sample, fewer sites are left to bind dsDNA IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The dsDNA IgG concentration in each sample is interpolated from this standard curve.

Intended Uses: This dsDNA IgG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse dsDNA IgG. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of anti-double stranded DNA, dsDNA IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7215353 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the anti-double stranded DNA, dsDNA IgG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing anti-double stranded DNA, dsDNA IgG. The ELISA analytical biochemical technique of the MBS7215353 kit is based on anti-double stranded DNA, dsDNA IgG antibody-anti-double stranded DNA, dsDNA IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect anti-double stranded DNA, dsDNA IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, anti-double stranded DNA, dsDNA IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).