Mouse Anti-Rat IgG1-AP
Référence MBS673737-1mL
Conditionnement : 1mL
Marque : MyBiosource
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Mouse Anti Rat IgG1 (Gamma 1 Chain Specific) Secondary Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673737MBS673737 is a secondary antibody targeting Mouse Anti Rat IgG1 (gamma 1 chain specific). Also known as Mouse Anti-Rat IgG1-AP. Reactive in Rat. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
Mouse Anti Rat IgG1 (gamma 1 chain specific), Secondary Antibody
Full Product Name
Mouse Anti-Rat IgG1-AP
Product Synonym Names
Mouse Anti-Rat IgG1- Alkaline Phosphatase-Labeled; Mouse Anti Rat IgG1 (gamma 1 chain specific)
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
G1 1C5
Host
Mouse
Species Reactivity
Rat
Form/Format
Alkaline Phosphatase (AP) Conjugate
Specificity
Reacts with the gamma 1 heavy chain (Fc) of Rat IgG1; may also react with other species.
Preparation and Storage
The purified antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added; store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg and the Cyanine 5 (Cy5) conjugate is supplied as 0.1mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The HRP conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The BIOT conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3; store at 2-8 degree C.
The R-PE conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent; store at 2-8 degree C.
FITC and R-PE conjugates should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg and the Cyanine 5 (Cy5) conjugate is supplied as 0.1mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The HRP conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The BIOT conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3; store at 2-8 degree C.
The R-PE conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent; store at 2-8 degree C.
FITC and R-PE conjugates should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested as a 2nd step reagent by flow cytometry and/or ELISA to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Product Categories/Family for Mouse Anti Rat IgG1 (gamma 1 chain specific) secondary antibody
Mouse Anti-Rat IgG1
Applications Tested/Suitable for Mouse Anti Rat IgG1 (gamma 1 chain specific) secondary antibody
ELISA; Immunofluorescence
Application Notes for Mouse Anti Rat IgG1 (gamma 1 chain specific) secondary antibody
Research Application(s): Identification and enumeration of IgG1 + cells by immunofluorescence microscopy
Second step reagent for rat IgG1 monoclonal antibodies
ELISA, can be used as capture antibody
ELISA Suggested Dilution: 1:2,000-1:4,000
Second step reagent for rat IgG1 monoclonal antibodies
ELISA, can be used as capture antibody
ELISA Suggested Dilution: 1:2,000-1:4,000
Other Notes
Small volumes of Mouse Anti Rat IgG1 (gamma 1 chain specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.