Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Mouse Anti-TET2 Monoclonal Antibody [Clone:CL6873]

Mouse Anti-TET2 Monoclonal Antibody [Clone:CL6873]

Imprimer Imprimer
Identifiez-vous

Référence AMAb91439

Conditionnement : 100ul

Marque : Atlas Antibodies

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

AMAb91439
Tet methylcytosine dioxygenase 2

Anti-TET2 Antibody

Monoclonal Antibody against HUMAN TET2

1.0 mg/ml
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
Anti-TET2 Antibody
info@biovalley.fr

Immunohistochemistry analysis in human placenta and pancreas tissues using AMAb91439 antibody. Corresponding TET2 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: Tet methylcytosine dioxygenase 2
  • Target gene: TET2
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: RAT - ENSRNOG00000023579 (84%)MOUSE - ENSMUSG00000040943 (89%)
  • Clonality: Monoclonal
  • Clone ID: CL6873
  • Isotype: IgG2b
  • Host: Mouse
  • Buffer: The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Protein A purified
  • Notes: The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: MAB-03660
  • Concentration: 1.0 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 2-10 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:20 - 1:50
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 1 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: Tet methylcytosine dioxygenase 2
  • Gene name: TET2
  • Alternative gene names: FLJ20032, KIAA1546, 
  • Ensembl: 
  • UniProt: 
  • Entrez: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

  • Fujikura K, Alruwaii ZI, Haffner MC, Trujillo MA, Roberts NJ, Hong SM, Macgregor-Das A, Goggins MG, Roy S, Meeker AK, Ding D, Wright M, He J, Hruban RH, Wood LDJ Pathol , 2021 May 21; 254(3):279-288. Epub 2021 May 212021 May 21
  • This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
    Human Protein Atlas

Vous serez peut-être également intéressé par les produits suivants :



Référence
Description
Cond.
Prix HT