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> TLR4 (Toll Like Receptor 4, CD284) (FITC)

TLR4 (Toll Like Receptor 4, CD284) (FITC)

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Référence T8050-34-FITC-100ul

Conditionnement : 100ul

Marque : US Biological

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T8050-34-FITC TLR4 (Toll Like Receptor 4, CD284) (FITC)

Clone Type
Monoclonal
Host
mouse
Source
human
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC IP
Crossreactivity
Bo Ca Gp Hu Mk Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.
TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS. Removal of sodium azide is recommended prior to use in functional assays.

Applications:
Suitable for use in Immunoprecipitation and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal working dilutions to be determined by researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 3F5 (HTA125)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Ba/F3 cell line expressing TLR4|Specificity: Recognizes human TLR4 (Toll Like Receptor 4). Has been shown to block the activation of monocytes with LPS. Species Crossreactivity: canine, rhesus monkey, guinea pig, porcine, bovine and mouse||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Ba/F3 cell line expressing TLR4
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human TLR4 (Toll Like Receptor 4). Has been shown to block the activation of monocytes with LPS. Species Crossreactivity: canine, rhesus monkey, guinea pig, porcine and bovine
References
1. Shimazu, R., et al., J. Exp. Med. 189: 1,777-1,782 (1999). 2. Jiang, Q., et al., J. Immunol. 165: 3,541-3,544 (2000). 3. Yang, S., et al., Infect. Immun. 69: 2,045-2,053 (2001). 4. Triantafilou, M., et al., J. Cell Sci. 115: 2,603-2,611 (2002).