Mouse Apolipoprotein E (APOE) ELISA Kit

Référence MBS2702317-48

Conditionnement : 48-Strip-Wells

Marque : MyBiosource

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Apolipoprotein E (APOE) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2702317
SPECIAL PROMOTIONAL PRICING for a limited time

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to APOE. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to APOE. Next, Avidin… More details

Product Overview

Online Datasheet in PDF
Product Name
Apolipoprotein E (APOE), ELISA Kit
Full Product Name
Mouse Apolipoprotein E (APOE) ELISA Kit
Product Gene Name
APOE elisa kit
Product Synonym Names
Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
Species Reactivity
Mouse
Samples
Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.6-1,000ng/mL
Sensitivity
< 5.7ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of APOE. No significant cross-reactivity or interference between APOE and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APOE were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APOE were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
36,154 Da
OMIM
M12529 mRNA
3D Structure
ModBase 3D Structure for P02649
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of APOE elisa kit

Mouse APOE ELISA Kit – Cat# MBS2702317

Other Notes

Small volumes of APOE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOE purchase

MBS2702317 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein E (APOE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOE. The ELISA analytical biochemical technique of the MBS2702317 kit is based on APOE antibody-APOE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for APOE. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
apolipoprotein E isoform b
NCBI Official Synonym Full Names
apolipoprotein E
NCBI Official Symbol
APOE
NCBI Official Synonym Symbols
AD2; LPG; APO-E; ApoE4; LDLCQ5
UniProt Protein Name
Apolipoprotein E
Protein Family
Apolipoprotein
UniProt Gene Name
APOE
UniProt Synonym Gene Names
Apo-E
NCBI Protein Information
apolipoprotein E

NCBI Summary for APOE

The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016]
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UniProt Comments for APOE

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with APOE elisa kit

 Reference Product  PubMed Publications
 LDLR elisa kit  >462 publications with APOE and LDLR
 APP elisa kit  >433 publications with APOE and APP
 SCARB1 elisa kit  >100 publications with APOE and SCARB1
 MAPT elisa kit  >82 publications with APOE and MAPT
 LRP1 elisa kit  >62 publications with APOE and LRP1
 A2M elisa kit  >62 publications with APOE and A2M
 PLTP elisa kit  >47 publications with APOE and PLTP
 VLDLR elisa kit  >44 publications with APOE and VLDLR
 PRNP elisa kit  >40 publications with APOE and PRNP
 LRP8 elisa kit  >13 publications with APOE and LRP8

Pathways associated with APOE elisa kit

 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 HDL-mediated Lipid Transport Pathway antibodies  HDL-mediated Lipid Transport Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with APOE elisa kit

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >5315 publications with APOE and Cardiovascular Diseases
 Alzheimer Disease Antibodies  >4934 publications with APOE and Alzheimer Disease
 Atherosclerosis Antibodies  >4115 publications with APOE and Atherosclerosis
 Arteriosclerosis Antibodies  >3499 publications with APOE and Arteriosclerosis
 Hyperlipidemias Antibodies  >1711 publications with APOE and Hyperlipidemias
 Inflammation Antibodies  >1227 publications with APOE and Inflammation
 Cognition Disorders Antibodies  >1082 publications with APOE and Cognition Disorders
 Coronary Disease Antibodies  >1041 publications with APOE and Coronary Disease
 Hypercholesterolemia Antibodies  >969 publications with APOE and Hypercholesterolemia
 Plaque, Atherosclerotic Antibodies  >949 publications with APOE and Plaque, Atherosclerotic

Organs/Tissues associated with APOE elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3719 publications with APOE and Brain
 Vascular Antibodies  >3484 publications with APOE and Vascular
 Liver Antibodies  >1673 publications with APOE and Liver
 Heart Antibodies  >1327 publications with APOE and Heart
 Muscle Antibodies  >1014 publications with APOE and Muscle
 Nerve Antibodies  >573 publications with APOE and Nerve
 Bone Antibodies  >481 publications with APOE and Bone
 Kidney Antibodies  >425 publications with APOE and Kidney
 Lung Antibodies  >229 publications with APOE and Lung
 Spleen Antibodies  >191 publications with APOE and Spleen