RayBio® C-Series Mouse Atherosclerosis Antibody Array 1 Kit. Detects 22 Mouse Atherosclerotic Factors. Suitable for all liquid sample types.
Mouse Atherosclerosis Array C1
Mouse Atherosclerosis Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 22 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
bFGF
| CD40 (TNFRSF5)
| Eotaxin-1 (CCL11)
| GCSF
| GM-CSF
|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-13
| L-Selectin (CD62L)
|
MCP-1 (CCL2)
| M-CSF
| MIP-3 alpha (CCL20)
| P-Selectin
| RANTES (CCL5)
|
TNF alpha
| VEGF-A
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Atherosclerosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
CCR5 facilitates endothelial progenitor cell recruitment and promotes the stabilization of atherosclerotic plaques in ApoE/ mice
- Species:
- Mouse
- Sample Type:
- Serum (Mice treated with splenectomy and APoE-/- mice)
- Year:
- 2015
- PubMed ID:
- 25889019
Green tea epigallocatechin-3-gallate attenuates Porphyromonas gingivalis-induced atherosclerosis
- Species:
- Mouse
- Sample Type:
- Serum (ApoE ko (atherosclerotic prone) mice treated with green tea)
- Year:
- 2013
- Journal Name:
- Pathogens and disease
- PubMed ID:
- 23620122
Oral challenge with Streptococcus sanguinis induces aortic inflammation and accelerates atherosclerosis in spontaneously hyperlipidemic mice
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2019
- Journal Name:
- Biochemical and Biophysical Research Communications
Porphyromonas gingivalis accelerates atherosclerosis in C57BL/6 mice fed a high-fat diet
- Species:
- Mouse
- Sample Type:
- Serum (Porphyromonas gingivalis in atherosclerosis model)
- Year:
- 2012
- Journal Name:
- Immunopharmacology and Immunotoxicology
- PubMed ID:
- 22047042