Mouse BALP (Bone Alkaline Phosphatase) CLIA Kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of BALP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to BALP. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BALP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BALP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of BALP. You can calculate the concentration of BALP in the samples by comparing the RLU value of the samples to the standard curve.

Small volumes of BALP clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2532866 is a CLIA kit targeting BALP. Also known as Mouse BALP (Bone Alkaline Phosphatase) CLIA Kit. Reactive in Mouse.