Mouse CD102/ICAM-2 Antibody : Biotin
Référence OASB00752
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse CD102/ICAM-2 Antibody : Biotin (OASB00752)
| Datasheets/Manuals | Printable datasheet for Mouse CD102/ICAM-2 Antibody : Biotin (OASB00752) |
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| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 3C4 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | Biotin |
| Application | FC, IHC-F, ICC, IP |
| Additional Information | Description: The 3C4 monoclonal antibody reacts with the cell surface glycoprotein mouse CD102 (ICAM-2), a ligand for the leucocyte integrin CD11a/CD18 (LFA-1). CD102 is constitutively expressed on T cells, B cells, and at high levels on vascular endothelial cells. It is also expressed on a variety of leukocyte cell lines. The 3C4 antibody blocks the interaction between ICAM-2 and LFA-1. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | COS cells transfected with mouse ICAM-2 cDNA and BW5147 AKR/J mouse thymoma cell line |
| Concentration | 0.5 mg/mL |
| Specificity | CD102 |
| Characterization | To ensure consistency in performance, each batch of product is tested by flow cytometry to conform to thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 1 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 1, In vitro blocking assays 1, Immunohistochemistry (frozen sections) 3, Immunoprecipitation 1 |
| Other Applications Data | Since applications vary, investigators are advised to determine theoptimum working dilution of the product that is appropriate for a specificapplication. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Xu, H., J.K. Bickford, E. Luther, C. Carpenito, F. Takei, and T.A. Springer. 1996. J. Immunol. 156:4909 2. Xu, H., O.L. Tong, A.R. deFougerolles, and T.A. Springer. 1992. J. Immunol. 149:2650 3. Majka, S.M., K.A. Jackson, K.A. Kienstra, M. W. Majesky, M.A. Goodell, and K.K. Hirschi. 2003. J. Clin. Invest 111(1):71. |
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| Gene Symbol | ICAM2 |
| Gene Full Name | Intercellular adhesion molecule 2 |
| Alias Symbols | Icam, CD102, Ly-60, Icam-2 |
| NCBI Gene Id | 15896 |
| Protein Name | intercellular adhesion molecule 2 |
| Uniprot ID | P35330 |
| Protein Accession # | NP_034624.1 |
| Nucleotide Accession # | NM_010494.2 |
